Homobifunctional Thiol Reactive – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:21:11 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Homobifunctional Thiol Reactive – VectorLabs https://staging.vectorlabs.com 32 32 Bis-Bromoacetamido-dPEG®₁₁ https://staging.vectorlabs.com/products/bis-bromoacetamido-dpeg11/ Sat, 24 Feb 2024 02:16:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=39402 Bis-Bromoacetamido-dPEG®11, product number QBD-11338, is a thiol-reactive, homobifunctional crosslinker, with the two reactive ends separated by a medium-length (44 atoms, 47.5 Å), single molecular weight, discrete polyethylene glycol (dPEG®) spacer. Both ends of the dPEG® spacer terminate with bromoacetyl groups that connect to the dPEG® crossbridge through amide bonds.

Bromoacetyl-functionalized reagents are useful alternatives to maleimide-functionalized reagents. The bromoacetyl group reacts with sulfhydryl groups at pH 8 or higher. Maleimide groups are chemoselective for thiols at pH 6.5 – 7.5 and lose selectivity at higher pH values. Bromoacetyl-thiol reaction rates are somewhat slower than maleimide-thiol reaction rates.

Specifications

Unit Size 50 mg, 1000 mg
Molecular Weight 786.54; single compound
Chemical formula C₂₈H₅₄Br₂N₂O₁₃
CAS N/A
Purity > 97%
Spacers dPEG® Spacer is 44 atoms and 47.5 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, DMSO, Acetonitrile, or DMAC
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-Bromoacetamido-dPEG®₁₁ appeared first on VectorLabs.

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Description

Bis-Bromoacetamido-dPEG®11, product number QBD-11338, is a thiol-reactive, homobifunctional crosslinker, with the two reactive ends separated by a medium-length (44 atoms, 47.5 Å), single molecular weight, discrete polyethylene glycol (dPEG®) spacer. Both ends of the dPEG® spacer terminate with bromoacetyl groups that connect to the dPEG® crossbridge through amide bonds.

Bromoacetyl-functionalized reagents are useful alternatives to maleimide-functionalized reagents. The bromoacetyl group reacts with sulfhydryl groups at pH 8 or higher. Maleimide groups are chemoselective for thiols at pH 6.5 – 7.5 and lose selectivity at higher pH values. Bromoacetyl-thiol reaction rates are somewhat slower than maleimide-thiol reaction rates.

Specifications

Unit Size50 mg, 1000 mg
Molecular Weight786.54; single compound
Chemical formulaC₂₈H₅₄Br₂N₂O₁₃
CASN/A
Purity> 97%
SpacersdPEG® Spacer is 44 atoms and 47.5 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, DMSO, Acetonitrile, or DMAC
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post Bis-Bromoacetamido-dPEG®₁₁ appeared first on VectorLabs.

]]> Bis-MAL-dPEG®₁₁ https://staging.vectorlabs.com/products/bis-mal-dpeg11/ Sat, 24 Feb 2024 02:10:20 +0000 https://staging.vectorlabs.com/?post_type=product&p=36129 Bis-MAL-dPEG®11, product number 10397, is a medium-length, homobifunctional, crosslinking reagent that links two molecules (e.g., peptides, proteins) together via the thiol-maleimide reaction (also known as the thiol-Michael addition). The ends of the molecule are both functionalized with maleimidopropionate groups. The two reactive ends are separated by a single molecular weight, discrete polyethylene glycol (dPEG®) spacer.

The thiol-maleimide reaction, a type of click chemistry reaction, is an extremely popular way to conjugate molecules. The maleimide functional group rapidly reacts with sulfhydryl groups, and the reaction is chemoselective for sulfhydryls in the pH range of 6.5 – 7.5.

The polyethylene glycol (PEG) spacer between the two maleimide groups is a single molecular weight compound with a discrete chain length (thus the tradename dPEG®). This product is not made from a disperse polymer but is instead a single compound. Applications for this product include, among many others, studying cell internalization of crosslinked proteins; developing FRET immunoassays; and anchoring biomolecules to surfaces.

Specifications

Unit Size 50 mg, 1000 mg
Molecular Weight 846.92; single compound
Chemical formula C₃₈H₆₂N₄O₁₇
CAS 854753-78-9
Purity > 98%
Spacers dPEG® Spacer is 49 atoms and 56.0 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-MAL-dPEG®₁₁ appeared first on VectorLabs.

]]>

Description

Bis-MAL-dPEG®11, product number 10397, is a medium-length, homobifunctional, crosslinking reagent that links two molecules (e.g., peptides, proteins) together via the thiol-maleimide reaction (also known as the thiol-Michael addition). The ends of the molecule are both functionalized with maleimidopropionate groups. The two reactive ends are separated by a single molecular weight, discrete polyethylene glycol (dPEG®) spacer.

The thiol-maleimide reaction, a type of click chemistry reaction, is an extremely popular way to conjugate molecules. The maleimide functional group rapidly reacts with sulfhydryl groups, and the reaction is chemoselective for sulfhydryls in the pH range of 6.5 – 7.5.

The polyethylene glycol (PEG) spacer between the two maleimide groups is a single molecular weight compound with a discrete chain length (thus the tradename dPEG®). This product is not made from a disperse polymer but is instead a single compound. Applications for this product include, among many others, studying cell internalization of crosslinked proteins; developing FRET immunoassays; and anchoring biomolecules to surfaces.

Specifications

Unit Size50 mg, 1000 mg
Molecular Weight846.92; single compound
Chemical formulaC₃₈H₆₂N₄O₁₇
CAS854753-78-9
Purity> 98%
SpacersdPEG® Spacer is 49 atoms and 56.0 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Kinetics of endophilin N-BAR domain dimerization and membrane interactions. Benjamin R. Capraro, Zheng Shi, Tingting Wu, Zhiming Chen, Joanna M. Dunn, Elizabeth Rhoades, Tobias Baumgart. Journal of Biological Chemistry. 2013, 288 (11) March 12, 2013. DOI: 10.1074/jbc.M112.435511.

Enhanced Fluorescence resonance energy transfer immunoassay with improved sensitivity based on the Fab’-based immunoconjugates. Yoshiyuki Ohiro, Hiroshi Ueda, Norio Shibata, Teruyuki Nagamune. Analytical Biochemistry. 2007, 360 (2) pp 266-272. January 15, 2007. DOI: 10.1016/j.ab.2006.10.025.

X-ray Photoelectron Spectroscopy and Differential Capacitance Study of Thiol-Functional Polysiloxane Films on Gold Supports. Patrick A. Johnson and Rastislav Levicky. Langmuir. 2004, 20 (22) pp 9621-9627. September 28, 2004. DOI: 10.1021/la048458s.

Polymercaptosiloxane Anchor Films for Robust Immobilization of Biomolecules to Gold Supports. Patrick A. Johnson and Rastislav Levicky. Langmuir . 2003, 19 (24) pp10288-10294. October 30, 2003. DOI: 10.1021/la035102s.

Unfolding Mechanisms and Conformational Stability of the Dimeric Endophilin N-BAR Domain. Rui Jin, Michael Grasso, Mingyang Zhou, Ronen Marmorstein, and Tobias Baumgart. ACS Omega. 2021, Volume 6 Issue 32. 08/04/2021. DOI: 10.1021/acsomega.1c01905.

The post Bis-MAL-dPEG®₁₁ appeared first on VectorLabs.

]]> Bis-MAL-dPEG®₃ https://staging.vectorlabs.com/products/bis-mal-dpeg3/ Sat, 24 Feb 2024 02:07:14 +0000 https://staging.vectorlabs.com/?post_type=product&p=35441 "Bis-MAL-dPEG®3, product number QBD-10215, is a short, homobifunctional, crosslinking reagent that links two molecules (e.g., peptides, proteins) together via the thiol-maleimide reaction (also known as the thiol-Michael addition). Maleimidopropionate groups functionalize each end of the molecule. A single molecular weight, discrete polyethylene glycol (dPEG®) spacer separates the two end groups.

The thiol-maleimide reaction, a type of click chemistry reaction, is a popular way to conjugate molecules. The maleimide functional group rapidly reacts with sulfhydryl groups, and the reaction is chemoselective for sulfhydryls in the pH range of 6.5 – 7.5. The discrete chain length polyethylene glycol (dPEG®) spacer between the two maleimide groups is a single molecular weight compound. Applications for this product include mapping which proteins in a complex are close together; determining ligand-receptor binding relationships; and construction of antibody-drug conjugates (ADCs), among many others."

Specifications

Unit Size 50 mg, 1000 mg
Molecular Weight 522.55; single compound
Chemical formula C₂₄H₃₄N₄O₉
CAS 756525-99-2
Purity > 98%
Spacers dPEG® Spacer is 28 atoms and 30 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-MAL-dPEG®₃ appeared first on VectorLabs.

]]>

Description

Bis-MAL-dPEG®3, product number QBD-10215, is a short, homobifunctional, crosslinking reagent that links two molecules (e.g., peptides, proteins) together via the thiol-maleimide reaction (also known as the thiol-Michael addition). Maleimidopropionate groups functionalize each end of the molecule. A single molecular weight, discrete polyethylene glycol (dPEG®) spacer separates the two end groups.

The thiol-maleimide reaction, a type of click chemistry reaction, is a popular way to conjugate molecules. The maleimide functional group rapidly reacts with sulfhydryl groups, and the reaction is chemoselective for sulfhydryls in the pH range of 6.5 – 7.5. The discrete chain length polyethylene glycol (dPEG®) spacer between the two maleimide groups is a single molecular weight compound. Applications for this product include mapping which proteins in a complex are close together; determining ligand-receptor binding relationships; and construction of antibody-drug conjugates (ADCs), among many others.

Specifications

Unit Size50 mg, 1000 mg
Molecular Weight522.55; single compound
Chemical formulaC₂₄H₃₄N₄O₉
CAS756525-99-2
Purity> 98%
SpacersdPEG® Spacer is 28 atoms and 30 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Backbone Degradable Multiblock N-(2-Hydroxypropyl)methacrylamide Copolymer Conjugates via Reversible Addition Fragmentation Chain Transfer Polymerization and Thiol-ene Coupling Reaction. Huaizhong Pan, Jiyuan Yang, Pavla Kopečková, and Jindřich Kopeček. Biomacromolecules. 2011, 12 (1) pp 247–252. January 10, 2011. DOI:10.1021/bm101254e.

Quantitative evaluation of the lengths of homobifunctional protein cross-linking reagents used as molecular rulers. NORA S. GREEN, EMIL REISLER, K.N. HOUK. Protein Science. 2001, 10 (7) pp 1293-1304. March 23, 2010. DOI: 10.1101/ps.51201.

Effects of modification at the fifth residue of l-conotoxin GIIIA with bulky tags on the electrically
stimulated contraction of the rat diaphragm. M. Nakamura, Y. Oba, H. Nakamura, Y. Ishida, T. Kohno, K. Sato. Journal Peptide Res. 2004, 64 (3) pp 110-117. May 30, 2004. DOI: 10.1111/j.1399-3011.2004.00175.x.

A Divalent Immunotoxin Formed by the Disulfide Bond between Hinge Regions of Fab Domain. SeongHyeok Choi, JiEun Kim, YongChan Lee, Young-Ju Jang, Ira Pastan, MuHyeon Choe. Bull Korean Chem.Soc. 2001, 22 (12) pp 1361-1365. September 3, 2001. DOI: www.pdf.lookchem.com/pdf/32/77fb36ea-eea2-4741-a825-b3234cd24299.pdf.

Mapping Protein-Protein Interactions between MutL and MutH by crosslinking. Luis Giron-Monzon, Laura Manelyte, Robert Ahrends, Dieter Kirsch, Bernhard Spengler, and Peter Friedhoff. J. Biol. Chem. 2004, 279 ( 47) pp 49338-49345. September 14, 2004. DOI: 10.1074/jbc.M409307200.

SWI/SNF- and RSC-Catalyzed Nucleosome Mobilization Requires Internal DNA Loop Translocation within Nucleosomes. Ning Liu, Craig L. Peterson and Jeffrey J. Hayes. Molecular and Cellular Biology. 2011, 31 (20), pp 4165-4177. August 22, 2011. DOI: 10.1128/MCB.05605-11.

Mitotic Spindle Assembly around RCC1-Coated Beads in Xenopus Egg Extracts. David Halpin, Petr Kalab, Jay Wang, Karsten Weis, Rebecca Heald. PLoS Bio. 2011, 9 (12) pp e1001225. December 27, 2011. DOI: 10.1371/journal.pbio.1001225.

Chemical Trapping of the Dynamic MutS-MutL Complex Formed in DNA Mismatch Repair in Escherichia coli. Ines Winkler, Andreas D. Marx, Damien Lariviere, Roger J. Heinze, Michele Cristovao, Annet Reumer, Ute Curth, Titia K. Sixma, and Peter Friedhoff. JBC. 2011, 286 (19) pp 17326–17337. May 13, 2011. DOI:10.1074/jbc.M110.187641.

Functional Characterization of Rhodopsin Monomers and Dimers in Detergents. Beata Jastrzebska, Tadao Maeda, Li Zhu, Dimitrios Fotiadis, Slawomir Filipek, Andreas Engel, Ronald E. Stenkamp, and Krzysztof Palczewskia. JBC. 2004, 279 (52) pp 54663-54675. December 24, 2004. DOI: 10.1074/jbc.M408691200.

Enhanced Fluorescence resonance energy transfer immunoassay with improved sensitivity based on the Fab’-based immunoconjugates. Yoshiyuki Ohiro, Hiroshi Ueda, Norio Shibata, Teruyuki Nagamune. Analytical Biochemistry. 2007, 360 (2) pp 266-272. January 15, 2007. DOI:10.1016/j.ab.2006.10.025.

Purification, characterization and cloning of a ricin B-like lectin from mushroom Clitocybe nebularis with antiproliferative activity against human leukemic T cells. Jure Pohleven, Nataša Obermajer, Jerica Sabotič, Sabina Anžlovar, Kristina Sepčić, Janko Kos , Bogdan Kralj, Borut Štrukelj, Jože Brzin. Biochimica et Biophysica Acta (BBA) – General Subjects. 2009, 1790 (3) pp 173-181. December 8, 2008. DOI: 10.1016/j.bbagen.2008.11.006.

Development of a homogeneous competitive immunoassay for phosphorylated protein antigen based on the enhanced fluorescence resonance energy transfer technology. Yoshiyuki Ohiro,1,⁎ Hiroshi Ueda,2,3 Norio Shibata,1 and Teruyuki Nagamune. Journal of Bioscience and Bioengineering. 2010, 109 (1), pp 15–19. July 29, 2009. DOI: 10.1016/j.jbiosc.2009.07.004.

Analysis of the Quaternary Structure of the MutL C-terminal Domain. Jan Kosinski, Ina Steindorf, Janusz M. Bujnicki, Luis Giron-Monzon and Peter Friedhoff. Journal of Molecular Biology. 2005, 351 (4) pp 895-909. August 26, 2005. DOI: 10.1016/j.jmb.2005.06.044.

X-ray Photoelectron Spectroscopy and Differential Capacitance Study of Thiol-Functional Polysiloxane Films on Gold Supports. Patrick A. Johnson and Rastislav Levicky. Langmuir. 2004, 20 (22) pp 9621-9627. September 28, 2004. DOI: 10.1021/la048458s.

Polymercaptosiloxane Anchor Films for Robust Immobilization of Biomolecules to Gold Supports. Patrick A. Johnson and Rastislav Levicky. Langmuir . 2003, 19 (24) pp10288-10294. October 30, 2003. DOI: 10.1021/la035102s.

X-ray photoelectron spectroscopy and infrared spectroscopy study of maleimide-activated supports for immobilization of oligodeoxyribonucleotides. Gang Shen, Maria Francis G. Anand and Rastislav Levicky. Nucleic Acids Research. 2004, 32 (20) pp 5973-5980. November 10, 2004. DOI: 10.1093/nar/gkh932.

Disulfide Sensitivity in the Env Protein Underlies Lytic Inactivation of HIV-1 by Peptide Triazole Thiols. Lauren D. Bailey, Ramalingam Venkat Kalyana Sundaram, Huiyuan Li, Caitlin Duffy, Rachna Aneja, Arangassery Rosemary Bastian, Andrew P. Holmes, Kantharaju Kamanna, Adel A. Rashad and Irwin Chaiken. ACS Chemical Biology. 2015, October 12, 2015. DOI: 10.1021/acschembio.5b00381.

In vivo mapping of a dynamic ribonucleoprotein granule interactome in early Drosophila embryos. Jimiao Zheng, Ming Gao, Nhan Huynh, Samuel J. Tindell, Hieu D. L. Vo, W. Hayes McDonald, and Alexey L. Arkov. Febs Open Bio. 2016, pp 1-9. October 3, 2016. DOI: 10.1002/2211-5463.12144.

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