Homobifunctional Amine Reactive – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:19:59 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Homobifunctional Amine Reactive – VectorLabs https://staging.vectorlabs.com 32 32 Bis-dPEG®₇-NHS ester https://staging.vectorlabs.com/products/bis-dpeg7-nhs-ester/ Sat, 24 Feb 2024 02:14:35 +0000 https://staging.vectorlabs.com/?post_type=product&p=37294 Bis-dPEG®7-NHS ester, product number QBD-10988, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a short (25 atoms, 28.6 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®7-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10988 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10988 is one in a line of similar products, Bis-dPEG®7-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®7-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 620.60; single compound
Chemical formula C₂₆H₄₀N₂O₁₅
CAS 1334170-02-3
Purity > 98%
Spacers dPEG® Spacer is 25 atoms and 28.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₇-NHS ester appeared first on VectorLabs.

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Description

Bis-dPEG®7-NHS ester, product number QBD-10988, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a short (25 atoms, 28.6 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®7-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10988 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10988 is one in a line of similar products, Bis-dPEG®7-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®7-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size100mg, 1000mg
Molecular Weight620.60; single compound
Chemical formulaC₂₆H₄₀N₂O₁₅
CAS1334170-02-3
Purity> 98%
SpacersdPEG® Spacer is 25 atoms and 28.6 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Inhibition of HLA-DQ2 Mediated Antigen Presentation by Analogues of a High Affinity 33-Residue Peptide from α2-Gliadin. Jiang Xia, Matthew Siegel, Elin Bergseng, Ludvig M. Sollid, and Chaitan Khosla. Journal of the American Chemical Society 2006 128 (6) pp 1859-1867 February 15, 2006. DOI:10.1021/ja056423o.

The post Bis-dPEG®₇-NHS ester appeared first on VectorLabs.

]]> Bis-dPEG®₁₇-NHS ester https://staging.vectorlabs.com/products/bis-dpeg17-nhs-ester/ Sat, 24 Feb 2024 02:14:34 +0000 https://staging.vectorlabs.com/?post_type=product&p=37286 Bis-dPEG®17-NHS ester, product number QBD-10979, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a long (55 atoms, 64.4 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®17-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10979 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10979 is one in a line of similar products, Bis-dPEG®17-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®17-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 1061.13; single compound
Chemical formula C₄₆H₈₀N₂O₂₅
CAS 1008402-79-6
Purity > 98%
Spacers dPEG® Spacer is 55 atoms and 64.4 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₁₇-NHS ester appeared first on VectorLabs.

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Description

Bis-dPEG®17-NHS ester, product number QBD-10979, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a long (55 atoms, 64.4 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®17-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10979 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10979 is one in a line of similar products, Bis-dPEG®17-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®17-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size100mg, 1000mg
Molecular Weight1061.13; single compound
Chemical formulaC₄₆H₈₀N₂O₂₅
CAS1008402-79-6
Purity> 98%
SpacersdPEG® Spacer is 55 atoms and 64.4 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Redox-Responsive Self-Assembly of Amphiphilic Multiblock Rod–Coil Polymers. Taek-Gyoung Kim, Chingu Kim, and Ji-Woong Park. Macromolecules. 2017, 50 (20) pp 8185–8191. 10/4/2017. DOI: 10.1021/acs.macromol.7b01650.

The post Bis-dPEG®₁₇-NHS ester appeared first on VectorLabs.

]]> Bis-dPEG®₂₅-NHS ester https://staging.vectorlabs.com/products/bis-dpeg25-nhs-ester/ Sat, 24 Feb 2024 02:14:33 +0000 https://staging.vectorlabs.com/?post_type=product&p=37278 Bis-dPEG®25-NHS ester, product number QBD-10968, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a long (79 atoms, 93.0 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®25-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10968 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10968 is one in a line of similar products, Bis-dPEG®25-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®25-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 1413.55; single compound
Chemical formula C₆₂H₁₁₂N₂O₃₃
CAS 1008402-79-6
Purity > 97%
Spacers dPEG® Spacer is 79 atoms and 93.0 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₂₅-NHS ester appeared first on VectorLabs.

]]>

Description

Bis-dPEG®25-NHS ester, product number QBD-10968, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a long (79 atoms, 93.0 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®25-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10968 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10968 is one in a line of similar products, Bis-dPEG®25-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®25-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size100mg, 1000mg
Molecular Weight1413.55; single compound
Chemical formulaC₆₂H₁₁₂N₂O₃₃
CAS1008402-79-6
Purity> 97%
SpacersdPEG® Spacer is 79 atoms and 93.0 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.

The post Bis-dPEG®₂₅-NHS ester appeared first on VectorLabs.

]]> Bis-dPEG®₂₁-NHS ester https://staging.vectorlabs.com/products/bis-dpeg21-nhs-ester/ Sat, 24 Feb 2024 02:14:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=37220 Bis-dPEG®21-NHS ester, product number QBD-10956, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a long (67 atoms, 79.1 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®21-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10956 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10956 is one in a line of similar products, Bis-dPEG®21-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®21-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 1237.34; single compound
Chemical formula C₅₄H₉₆N₂O₂₉
CAS 1008402-79-6
Purity > 97%
Spacers dPEG® Spacer is 67 atoms and 79.1 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₂₁-NHS ester appeared first on VectorLabs.

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Description

Bis-dPEG®21-NHS ester, product number QBD-10956, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a long (67 atoms, 79.1 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®21-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10956 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10956 is one in a line of similar products, Bis-dPEG®21-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®21-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size100mg, 1000mg
Molecular Weight1237.34; single compound
Chemical formulaC₅₄H₉₆N₂O₂₉
CAS1008402-79-6
Purity> 97%
SpacersdPEG® Spacer is 67 atoms and 79.1 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Redox-Responsive Self-Assembly of Amphiphilic Multiblock Rod–Coil Polymers. Taek-Gyoung Kim, Chingu Kim, and Ji-Woong Park. Macromolecules. 2017, 50 (20) pp 8185–8191. 10/4/2017. DOI: 10.1021/acs.macromol.7b01650.

The post Bis-dPEG®₂₁-NHS ester appeared first on VectorLabs.

]]> Bis-dPEG®₁₃-NHS ester https://staging.vectorlabs.com/products/bis-dpeg13-nhs-ester/ Sat, 24 Feb 2024 02:13:53 +0000 https://staging.vectorlabs.com/?post_type=product&p=37211 Bis-dPEG®13-NHS ester, product number QBD-10954, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a medium-length (43 atoms, 50.1 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®13-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10954 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10954 is one in a line of similar products, Bis-dPEG®13-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®13-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 884.92; single compound
Chemical formula C₃₈H₆₄N₂O₂₁
CAS 1008402-79-6
Purity > 98%
Spacers dPEG® Spacer is 43 atoms and 50.1 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₁₃-NHS ester appeared first on VectorLabs.

]]>

Description

Bis-dPEG®13-NHS ester, product number QBD-10954, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a medium-length (43 atoms, 50.1 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®13-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10954 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10954 is one in a line of similar products, Bis-dPEG®13-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®13-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size100mg, 1000mg
Molecular Weight884.92; single compound
Chemical formulaC₃₈H₆₄N₂O₂₁
CAS1008402-79-6
Purity> 98%
SpacersdPEG® Spacer is 43 atoms and 50.1 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

 



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Redox-Responsive Self-Assembly of Amphiphilic Multiblock Rod–Coil Polymers. Taek-Gyoung Kim, Chingu Kim, and Ji-Woong Park. Macromolecules. 2017, 50 (20) pp 8185–8191. 10/4/2017. DOI: 10.1021/acs.macromol.7b01650.

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.

The post Bis-dPEG®₁₃-NHS ester appeared first on VectorLabs.

]]> Bis-dPEG®₃-NHS ester https://staging.vectorlabs.com/products/bis-dpeg3-nhs-ester/ Sat, 24 Feb 2024 02:12:06 +0000 https://staging.vectorlabs.com/?post_type=product&p=36720 Bis-dPEG®3-NHS ester, product number QBD-10726, is a homobifunctional, amine-reactive, crosslinking reagent. Each end of the short (13 atoms, 14.6 Å), single molecular weight, discrete PEG (dPEG®) linker is functionalized as the N-hydroxysuccinimidyl (NHS) ester of a propionic acid group. The optimal pH range for reacting the end groups is 7.0 - 7.5. The product is soluble in water and many organic solvents; however, in water, it hydrolyzes rapidly. Therefore, we advise against storing aqueous stock solutions of this product. Rather, unused material in aqueous media should be discarded promptly.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 444.39; single compound
Chemical formula C₁₈H₂₄N₂O₁₁
CAS 1314378-16-9
Purity > 98%
Spacers dPEG® Spacer is 13 atoms and 14.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₃-NHS ester appeared first on VectorLabs.

]]>

Description

Bis-dPEG®3-NHS ester, product number QBD-10726, is a homobifunctional, amine-reactive, crosslinking reagent. Each end of the short (13 atoms, 14.6 Å), single molecular weight, discrete PEG (dPEG®) linker is functionalized as the N-hydroxysuccinimidyl (NHS) ester of a propionic acid group. The optimal pH range for reacting the end groups is 7.0 – 7.5. The product is soluble in water and many organic solvents; however, in water, it hydrolyzes rapidly. Therefore, we advise against storing aqueous stock solutions of this product. Rather, unused material in aqueous media should be discarded promptly.

Specifications

Unit Size100mg, 1000mg
Molecular Weight444.39; single compound
Chemical formulaC₁₈H₂₄N₂O₁₁
CAS1314378-16-9
Purity> 98%
SpacersdPEG® Spacer is 13 atoms and 14.6 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Bis-dPEG®₃-NHS ester appeared first on VectorLabs.

]]> Bis-dPEG®₂-NHS ester https://staging.vectorlabs.com/products/bis-dpeg2-nhs-ester/ Sat, 24 Feb 2024 02:12:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=36712 Bis-dPEG®2-NHS ester, product number QBD-10724, is a homobifunctional, amine-reactive, crosslinking reagent. Each end of the short (10 atoms, 11.0 Å), single molecular weight, discrete PEG (dPEG®) linker is functionalized as the N-hydroxysuccinimidyl (NHS) ester of a propionic acid group. The optimal pH range for reacting the end groups is 7.0 - 7.5. The product is soluble in water and many organic solvents; however, in water, it hydrolyzes rapidly. Therefore, we advise against storing aqueous stock solutions of this product. Rather, unused material in aqueous media should be discarded promptly.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 400.34; single compound
Chemical formula C₁₆H₂₀N₂O₁₀
CAS 65869-63-8
Purity > 98%
Spacers dPEG® Spacer is 10 atoms and 11.0 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₂-NHS ester appeared first on VectorLabs.

]]>

Description

Bis-dPEG®2-NHS ester, product number QBD-10724, is a homobifunctional, amine-reactive, crosslinking reagent. Each end of the short (10 atoms, 11.0 Å), single molecular weight, discrete PEG (dPEG®) linker is functionalized as the N-hydroxysuccinimidyl (NHS) ester of a propionic acid group. The optimal pH range for reacting the end groups is 7.0 – 7.5. The product is soluble in water and many organic solvents; however, in water, it hydrolyzes rapidly. Therefore, we advise against storing aqueous stock solutions of this product. Rather, unused material in aqueous media should be discarded promptly.

Specifications

Unit Size100mg, 1000mg
Molecular Weight400.34; single compound
Chemical formulaC₁₆H₂₀N₂O₁₀
CAS65869-63-8
Purity> 98%
SpacersdPEG® Spacer is 10 atoms and 11.0 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.

The post Bis-dPEG®₂-NHS ester appeared first on VectorLabs.

]]> Bis-dPEG®₉-NHS ester https://staging.vectorlabs.com/products/bis-dpeg9-nhs-ester/ Sat, 24 Feb 2024 02:07:35 +0000 https://staging.vectorlabs.com/?post_type=product&p=35573 Bis-dPEG®9-NHS ester, product number QBD-10246, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a medium-length (31 atoms, 35.7 Å), discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Several scientific publications describe the use of QBD-10246.

NHS esters react with free amines such as the ε-amines of lysine with an optimal pH range of 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.0. NHS esters are susceptible to hydrolysis in aqueous media. The hydrolysis rate of the ester increases with the pH.

Thus, we strongly discourage storing Bis-dPEG®9-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 708.71; single compound
Chemical formula C₃₀H₄₈N₂O₁₇
CAS 1008402-79-6
Purity > 97%
Spacers dPEG® Spacer is 31 atoms and 35.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₉-NHS ester appeared first on VectorLabs.

]]>

Description

Bis-dPEG®9-NHS ester, product number QBD-10246, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a medium-length (31 atoms, 35.7 Å), discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Several scientific publications describe the use of QBD-10246.

NHS esters react with free amines such as the ε-amines of lysine with an optimal pH range of 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.0. NHS esters are susceptible to hydrolysis in aqueous media. The hydrolysis rate of the ester increases with the pH.

Thus, we strongly discourage storing Bis-dPEG®9-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight708.71; single compound
Chemical formulaC₃₀H₄₈N₂O₁₇
CAS1008402-79-6
Purity> 97%
SpacersdPEG® Spacer is 31 atoms and 35.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Hermanson, G. T. Chapter 5, Homobifunctional Crosslinkers. In Bioconjugate Techniques, 3rd edition; Academic Press: New York, NY, 2013; pp 275–298.

Potent D-peptide inhibitors of HIV-1 entry. Brett D. Welch, Andrew P. VanDemark, Annie Heroux, Christopher P. Hill, and Michael S. Kay. PNAS. 2007, 104 (43) pp 16828-16833. October 23, 2007. DOI: 10.1073/pnas.0708109104.

Dynamic electromagnetophoretic force analysis of a single binding interaction between lectin and mannan polysaccharide on yeast cell surface. Yoshinori Iigunia and Hitoshi Watarai. Analyst. 2010, 135 (6) pp 1426–1432. March 16, 2010. DOI: 10.1039/b924339a.

Orientation-regulated immobilization of Jagged1 on glass substrates for ex vivo proliferation of a bone marrow cell population containing hematopoietic stem cells. Hiroyuki Toda, Masaya Yamamoto, Hiroshi Kohara, Yasuhiko Tabata. Biomaterials. 2011, 32 (29) pp 6920-6928. October 2011. DOI: 10.1016/j.biomaterials.2011.05.093.

Cyclic and dimeric gluten peptide analogues inhibiting DQ2-mediated antigen presentation in celiac disease. Jiang Xia, Elin Bergseng, Burkhard Fleckenstein, Matthew Siegel, Chu-Young Kim, Chaitan Khosla and Ludvig M. Sollid. Bioorganic & Medicinal Chemistry. 2007, 15 (20) pp 6565–6573. October 15, 2007. DOI: 10.1016/j.bmc.2007.07.001.

Phosphoramidate and phosphate prodrugs of (_)-b-D-(2R,4R)-dioxolane-thymine: Synthesis, anti-HIV activity and stability studies. Yuzeng Liang, Janarthanan Narayanasamy, Raymond F. Schinazib and Chung K. Chua. Bioorganic & Medicinal Chemistry. 2006, 14 (7) pp 2178–2189. April 1, 2006. DOI: 10.1016/j.bmc.2005.11.008.

Mapping of Molecular Structure of the Nanoscale Surface in Bio-nanoparticles. Luciana M Herda, Delyan R Hristov, Maria Cristina Lo Giudice, Ester Polo, and Kenneth A Dawson. Journal of the American Chemical Society. 2017. 139(1), 111-114. DOI: 10.1021/jacs.6b12297.

The post Bis-dPEG®₉-NHS ester appeared first on VectorLabs.

]]> Bis-dPEG®₅-acid https://staging.vectorlabs.com/products/bis-dpeg5-acid/ Sat, 24 Feb 2024 02:07:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=35515 "Bis-dPEG®5-acid, product number QBD-10230, is a short, monodisperse, homobifunctional polyethylene glycol (PEG) compound designed for crosslinking amines. Two propionic acid groups terminate either end of a discrete PEG (dPEG®) chain. These two end groups can be coupled directly to amines using a carbodiimide such as EDC. Alternatively, the carboxylate ends can be functionalized as active esters. Reactive groups such as NHS, TFP, PFP, and HOBt are suitable for this purpose. Reaction with various amines follows the activation step.

The hydrophilic dPEG® spacer imparts water solubility and flexibility to molecules to which it is conjugated. Moreover, conjugates containing dPEG® moieties have increased hydrodynamic volume. Also, the non-immunogenic crosslinker reduces or eliminates the antigenicity of the resulting conjugates.

Peptides, proteins, amine-containing biomolecules, and small molecules with primary or secondary amine groups can all be crosslinked with Bis-dPEG®5-acid. It may also be useful for peptide stapling or as a building block for macromolecular constructs."

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 338.35; single compound
Chemical formula C₁₄H₂₆O₉
CAS 439114-13-3
Purity > 98%
Spacers dPEG® Spacer is 19 atoms and 21.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₅-acid appeared first on VectorLabs.

]]>

Description

Bis-dPEG®5-acid, product number QBD-10230, is a short, monodisperse, homobifunctional polyethylene glycol (PEG) compound designed for crosslinking amines. Two propionic acid groups terminate either end of a discrete PEG (dPEG®) chain. These two end groups can be coupled directly to amines using a carbodiimide such as EDC. Alternatively, the carboxylate ends can be functionalized as active esters. Reactive groups such as NHS, TFP, PFP, and HOBt are suitable for this purpose. Reaction with various amines follows the activation step.

The hydrophilic dPEG® spacer imparts water solubility and flexibility to molecules to which it is conjugated. Moreover, conjugates containing dPEG® moieties have increased hydrodynamic volume. Also, the non-immunogenic crosslinker reduces or eliminates the antigenicity of the resulting conjugates.

Peptides, proteins, amine-containing biomolecules, and small molecules with primary or secondary amine groups can all be crosslinked with Bis-dPEG®5-acid. It may also be useful for peptide stapling or as a building block for macromolecular constructs.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight338.35; single compound
Chemical formulaC₁₄H₂₆O₉
CAS439114-13-3
Purity> 98%
SpacersdPEG® Spacer is 19 atoms and 21.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Bis-dPEG®₅-acid appeared first on VectorLabs.

]]> Bis-dPEG®₅-NHS ester https://staging.vectorlabs.com/products/bis-dpeg5-nhs-ester/ Sat, 24 Feb 2024 02:07:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=35489 Bis-dPEG®5-NHS ester, product number QBD-10224, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a short, discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®5-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. Various uses for Bis-dPEG®5-NHS ester have been published in the scientific literature.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®5-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 532.50; single compound
Chemical formula C₂₂H₃₂N₂O₁₃
CAS 756526-03-1
Purity > 97%
Spacers dPEG® Spacer is 19 atoms and 21.568 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₅-NHS ester appeared first on VectorLabs.

]]>

Description

Bis-dPEG®5-NHS ester, product number QBD-10224, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a short, discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®5-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. Various uses for Bis-dPEG®5-NHS ester have been published in the scientific literature.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®5-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight532.50; single compound
Chemical formulaC₂₂H₃₂N₂O₁₃
CAS756526-03-1
Purity> 97%
SpacersdPEG® Spacer is 19 atoms and 21.568 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Cyclic and dimeric gluten peptide analogues inhibiting DQ2-mediated antigen presentation in celiac disease. Jiang Xia, Elin Bergseng, Burkhard Fleckenstein, Matthew Siegel, Chu-Young Kim, Chaitan Khosla and Ludvig M. Sollid. Bioorganic & Medicinal Chemistry. 15 (2007) pp 6565–6573. July 25, 2007. DOI: 10.1016/j.bmc.2007.07.001.

Design of a Potent D-Peptide HIV-1 Entry Inhibitor with a Strong Barrier to Resistance. Brett D. Welch, J. Nicholas Francis, Joseph S. Redman, Suparna Paul, Matthew T. Weinstock, Jacqueline D. Reeves, Yolanda S. Lie, Frank G. Whitby, Debra M. Eckert, Christopher P. Hill, Michael J. Root, and Michael S. Kay. Journal of Virology. 2010, 84 (21) pp 11235–11244. November 2010. DOI: 10.1128/JVI.01339-10.

Ligand-regulated oligomerization of b2-adrenoceptors in a model lipid bilayer. Juan Jose´ Fung, Xavier Deupi, Leonardo Pardo, Xiao Jie Yao, Gisselle A Velez-Ruiz, Brian T DeVree, Roger K Sunahara and Brian K Kobilka. The EMBO Journal, 2009, 28, pp 3315–3328. September 17, 2009. DOI: 10.1038/emboj.2009.267.

Monitoring the switching of single BSA-ATTO 488 molecules covalently end-attached to a pH responsive PAA brush. Namik Akkilic, Robert Molenaar, Mireille M.A.E. Claessens, Christian Blum, and Wiebe M. de Vos. Langmuir. 2016, pp 1-10. August 15, 2016. DOI: 10.1021/acs.langmuir.6b01064.

G-Quadruplex Induction by the Hairpin Pyrrole–Imidazole Polyamide Dimer. Shunsuke Obata, Sefan Asamitsu, Kaori Hashiya, Toshikazu Bando, and Hiroshi Sugiyama. Biochemistry. 2018, 57, pp 498-502. December 13, 2017. DOI: 10.1021/acs.biochem.7b01059.

Surface Modification on Acoustic Wave Biosensors for Enhanced Specificity. Onursal Onen, Asad A. Ahmad, Rasim Guldiken and Nathan D. Gallant. Sensors. 2012, 12 pp 12317-12328. September 10, 2012. DOI: 10.3390/s120912317.

Surface Functionalization and Analysis Thereof for an Ovarian Cancer Diagnostic Biosensor. Asad Ali Ahmad. USF Scholor Commons. 2011, 1 (1) pp 297 416. June 21, 2011. DOI: scholarcommons.usf.edu/etd/2977.

Serum albumin ‘camouflage’ of plant virus based nanoparticles prevents their antibody recognition and enhances pharmacokinetics. Andrzej S. Pitek, Slater A. Jameson, Frank A. Veliz, Sourabh Shukla, and Nicole F. Steinmetz. Biomaterials. 2016, 89 pp 89-97, February 23, 2016. DOI: 10.1016/j.biomaterials.2016.02.032.

Interdomain regulation of the ATPase activity of the ABC transporter hemolysin B from E. coli. Sven Reimann, Gereon Poschmann, Kerstin Kanonenbera, Kai Stuhler, Sander H.J. Smits, and Lutz Schmitt. Biochemical Journal. 2016, June 8, 2016. DOI: 10.1042/BCJ20160154.

Redox-Responsive Self-Assembly of Amphiphilic Multiblock Rod–Coil Polymers. Taek-Gyoung Kim, Chingu Kim, and Ji-Woong Park. Macromolecules. 2017, 50 (20) pp 8185–8191. 10/4/2017. DOI: 10.1021/acs.macromol.7b01650.

Toward the Development of GE11-Based Radioligands for Imaging of Epidermal Growth Factor Receptor-Positive Tumors. Benedikt Judmann, Diana Braun, Ralf Schirrmacher, Björn Wängler, Gert Fricker, and Carmen Wängler. ACS Omega 2022, 7, 31, 27690–27702. 2022. https://doi.org/10.1021/acsomega.2c03407

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.

The post Bis-dPEG®₅-NHS ester appeared first on VectorLabs.

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