Heterobifunctional Hydrazone/Oxime Bond Formation – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:16:00 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Heterobifunctional Hydrazone/Oxime Bond Formation – VectorLabs https://staging.vectorlabs.com 32 32 4-formyl-benzamido-dPEG®₂₄-TFP ester https://staging.vectorlabs.com/products/4-formyl-benzamido-dpeg24-tfp-ester/ Sat, 24 Feb 2024 02:04:48 +0000 https://staging.vectorlabs.com/?post_type=product&p=34999 4-formyl-benzamido-dPEG®24-TFP ester, product number 10082, is useful for introducing an aryl aldehyde into a peptide, protein, or other biomolecules. The 2,3,5,6-tetrafluorophenyl (TFP) ester end reacts with free amines, while the flexible, non-immunogenic, discrete PEG (dPEG®) spacer adds water solubility to the conjugate. Reacting the aldehyde moiety with a hydrazide or aminooxy reactive group forms hydrazone or oxime bonds, respectively.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 1426.53; single compound
Chemical formula C₆₅H₁₀₇F₄NO₂₈
CAS N/A
Purity > 97%
Spacers dPEG® Spacer is 83 atoms and 93.0 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) DMF, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post 4-formyl-benzamido-dPEG®₂₄-TFP ester appeared first on VectorLabs.

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Description

4-formyl-benzamido-dPEG®24-TFP ester, product number QBD-10082, is useful for introducing an aryl aldehyde into a peptide, protein, or other biomolecules. The 2,3,5,6-tetrafluorophenyl (TFP) ester end reacts with free amines, while the flexible, non-immunogenic, discrete PEG (dPEG®) spacer adds water solubility to the conjugate. Reacting the aldehyde moiety with a hydrazide or aminooxy reactive group forms hydrazone or oxime bonds, respectively.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 1426.53; single compound
Chemical formula C₆₅H₁₀₇F₄NO₂₈
CAS N/A
Purity > 97%
Spacers dPEG® Spacer is 83 atoms and 93.0 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) DMF, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

  2. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

  3. Strategies for Neoglycan Conjugation to Human Acid r-Glucosidase. Qun Zhou, James E. Stefano, John Harrahy, Patrick Finn, Luis Avila, Josephine Kyazike, Ronnie Wei, Scott M. Van Patten, Russell Gotschall, Xiaoyang Zheng, Yunxiang Zhu, Tim Edmunds, and Clark Q. Pan. Bioconjugate Chem. 2011, 22 pp 741–751. March 18, 2011. doi.org/10.1021/bc1005416

  4. Single-Step Conjugation of Antibodies to Quantum Dots for Labeling Cell Surface Receptors in Mammalian Cells. Gopal Iyer, Jianmin Xu, and Shimon Weiss. Bioconjugation Protocols. 2011, 751 pp 553-563. January 2, 2011. DOI: 10.1007/978-1-61779-151-2-34.

The post 4-formyl-benzamido-dPEG®₂₄-TFP ester appeared first on VectorLabs.

]]> 4-formyl-benzamido-dPEG®₁₂-TFP ester https://staging.vectorlabs.com/products/4-formyl-benzamido-dpeg12-tfp-ester/ Sat, 24 Feb 2024 02:04:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=34964 4-formyl-benzamido-dPEG®12-TFP ester, product number 10081, is useful for introducing an aryl aldehyde into a peptide, protein, or other biomolecules. The 2,3,5,6-tetrafluorophenyl (TFP) ester end reacts with free amines, while the flexible, non-immunogenic, discrete PEG (dPEG®) spacer adds water solubility to the conjugate. Reacting the aldehyde moiety with a hydrazide or aminooxy reactive group forms hydrazone or oxime bonds, respectively.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 897.90; single compound
Chemical formula C₄₁H₅₉F₄NO₁₆
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 46 atoms and 49.3 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) DMF, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post 4-formyl-benzamido-dPEG®₁₂-TFP ester appeared first on VectorLabs.

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Description

4-formyl-benzamido-dPEG®12-TFP ester, product number QBD-10081, is useful for introducing an aryl aldehyde into a peptide, protein, or other biomolecules. The 2,3,5,6-tetrafluorophenyl (TFP) ester end reacts with free amines, while the flexible, non-immunogenic, discrete PEG (dPEG®) spacer adds water solubility to the conjugate. Reacting the aldehyde moiety with a hydrazide or aminooxy reactive group forms hydrazone or oxime bonds, respectively.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 897.90; single compound
Chemical formula C₄₁H₅₉F₄NO₁₆
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 46 atoms and 49.3 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) DMF, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

  2. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

  3. Strategies for Neoglycan Conjugation to Human Acid r-Glucosidase. Qun Zhou, James E. Stefano, John Harrahy, Patrick Finn, Luis Avila, Josephine Kyazike, Ronnie Wei, Scott M. Van Patten, Russell Gotschall, Xiaoyang Zheng, Yunxiang Zhu, Tim Edmunds, and Clark Q. Pan. Bioconjugate Chem. 2011, 22 pp 741–751. March 18, 2011. doi.org/10.1021/bc1005416.

  4. Single-Step Conjugation of Antibodies to Quantum Dots for Labeling Cell Surface Receptors in Mammalian Cells. Gopal Iyer, Jianmin Xu, and Shimon Weiss. Bioconjugation Protocols. 2011, 751 pp 553-563. January 2, 2011. DOI: 10.1007/978-1-61779-151-2_34.

The post 4-formyl-benzamido-dPEG®₁₂-TFP ester appeared first on VectorLabs.

]]> 4-formyl-benzamido-dPEG®₁₂-EDA-MAL https://staging.vectorlabs.com/products/4-formyl-benzamido-dpeg12-eda-mal/ Sat, 24 Feb 2024 02:04:46 +0000 https://staging.vectorlabs.com/?post_type=product&p=34955 4-formyl-benzamido-dPEG®12-EDA-MAL, product number QBD-10075, is a medium-length (57 atoms, 59.6 Å) single molecular weight, discrete PEG (dPEG®) spacer functionalized with an aryl aldehyde on one end and a malemide reactive group on the other. It is designed for the stable crosslinking of biomolecules. The benzaldehyde group forms hydrazone bonds with hydrazine and hydrazide groups and oxime bonds with aldehydes and ketones, while the maleimide reactive group forms thioether bonds with free sulfhydryl groups.

Hydrazone bonds are acid-labile, allowing for constructs that self-disassemble in lysosomes, while oxime bonds are much more stable. The maleimide group allows for conjugation to free thiols on proteins and peptides. The single molecular weight dPEG® linker has no dispersity, which simplifies analytical considerations compared to polymer PEGs where the broad range of PEG chain lengths and molecular weights complicate the analysis of the conjugates.

The potential uses for this molecule include supramolecular construction of drug delivery systems and imaging applications.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 943.04; single compound
Chemical formula C₄₄H₇₀N₄O₁₈
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 57 atoms and 59.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Acetonitrile, DMSO or Methanol.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post 4-formyl-benzamido-dPEG®₁₂-EDA-MAL appeared first on VectorLabs.

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Description

4-formyl-benzamido-dPEG®12-EDA-MAL, product number QBD-10075, is a medium-length (57 atoms, 59.6 Å) single molecular weight, discrete PEG (dPEG®) spacer functionalized with an aryl aldehyde on one end and a malemide reactive group on the other. It is designed for the stable crosslinking of biomolecules. The benzaldehyde group forms hydrazone bonds with hydrazine and hydrazide groups and oxime bonds with aldehydes and ketones, while the maleimide reactive group forms thioether bonds with free sulfhydryl groups.

Hydrazone bonds are acid-labile, allowing for constructs that self-disassemble in lysosomes, while oxime bonds are much more stable. The maleimide group allows for conjugation to free thiols on proteins and peptides. The single molecular weight dPEG® linker has no dispersity, which simplifies analytical considerations compared to polymer PEGs where the broad range of PEG chain lengths and molecular weights complicate the analysis of the conjugates.

The potential uses for this molecule include supramolecular construction of drug delivery systems and imaging applications.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 943.04; single compound
Chemical formula C₄₄H₇₀N₄O₁₈
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 57 atoms and 59.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Acetonitrile, DMSO or Methanol.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post 4-formyl-benzamido-dPEG®₁₂-EDA-MAL appeared first on VectorLabs.

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