Description

MAL-dPEG®24-amido-dPEG24-amido-dPEG®24-DSPE, product number QBD-11388, is designed to facilitate payload delivery in liposomes and micelles. The long (234 atoms, 275.8 Å), single molecular weight, discrete-chain-length polyethylene glycol (dPEG®) spacer is conjugated to 1, 2-Distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) for liposomal or micellar insertion and functionalized with a sulfhydryl-reactive maleimidopropyl moiety that forms thioether bonds with a payload (e.g., a targeting peptide or cytotoxin) containing a free thiol group. The free maleimide group may also facilitate cellular uptake of liposomes or micelles containing the moiety through thiol-mediated membrane trafficking.

Unlike traditional polymer PEG products, which consist of an intractable mixture of different chain lengths and molecular weights of PEG and have an irreproducible purity profile, Vector Laboratories dPEG® products are high purity single molecules containing one PEG chain length and molecular weight with a reproducible purity profile.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Description

DBCO-dPEG®24-amido-dPEG®24-DSPE is a 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) group functionalized with a long (152 atoms, 180.0 Å) single molecular weight, discrete PEG (dPEG®) spacer. The spacer consists of two dPEG®24 linkers joined by an amide bond, making it effectively a dPEG®48 spacer with a molecular weight slightly greater than 2,000 Daltons (that is, equivalent to a PEG2000 spacer). The distal end of the dPEG® spacer is functionalized with a dibenzylcycloctyne (DBCO) group for strain-promoted, azide-alkyne cylcoaddition (SPAAC) reaction, also known as copper-free click chemistry. The DBCO can be functionalized with a suitable cytotoxic or imaging payload. The DSPE group is designed for insertion into liposomes, micelles, or other lipid nanoparticles.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Description

MAL-dPEG®24-amido-dPEG24-DSPE, product number QBD-11093, is designed to facilitate payload delivery (e.g., a targeting peptide or cytotoxin) in liposomes and micelles. The long (158 atoms, 150.4 Å), single molecular weight, discrete-chain-length polyethylene glycol (dPEG®) spacer is conjugated to 1, 2-Distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) for liposomal or micellar insertion and functionalized with a sulfhydryl-reactive maleimidopropyl moiety that forms thioether bonds with a payload containing a free thiol group. The free maleimide group may also facilitate cellular uptake of liposomes or micelles containing the moiety through thiol-mediated membrane trafficking.

Unlike traditional polymer PEG products, which consist of an intractable mixture of different chain lengths and molecular weights of PEG and have an irreproducible purity profile, Vector Laboratories dPEG® products are high purity single molecules containing one PEG chain length and molecular weight with a reproducible purity profile.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Description

MAL-dPEG®12-DSPE, product number QBD-11028, is designed to facilitate payload delivery (e.g., a targeting peptide or cytotoxin) in liposomes and micelles. The medium-length (46 atoms, 53.3 Å), single molecular weight, discrete-chain-length polyethylene glycol (dPEG®) spacer is conjugated to 1, 2-Distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) for liposomal or micellar insertion and functionalized with a sulfhydryl-reactive maleimidopropyl moiety that forms thioether bonds with a payload containing a free thiol group. The free maleimide group may also facilitate cellular uptake of liposomes or micelles containing the moiety through thiol-mediated membrane trafficking.

Unlike traditional polymer PEG products, which consist of an intractable mixture of different chain lengths and molecular weights of PEG and have an irreproducible purity profile, Vector Laboratories dPEG® products are high purity single molecules containing one PEG chain length and molecular weight with a reproducible purity profile.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Description

NHS-dPEG®13-DSPE, product number QBD-10028, is designed for use in liposomes and micelles. The lipophilic end of the molecule is the lipid 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) modified by conjugation to a single molecular weight, discrete polyethylene glycol (dPEG®) chain. By design, the dPEG® spacer provides discrete PEG surface coating for liposomes and micelles, protecting them from opsonization and removal by the RES. The dPEG® spacer is functionalized as an N-hydroxysuccinimide (NHS) ester, which reacts with primary or secondary amines to form amide bonds, allowing for surface modification of a liposome or micelle with peptides, proteins, or small molecule drugs.

Specifications

Documents

References

1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.