Methyltetrazine-PEG4-STP Ester is an amine-reactive, water-soluble labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers in aqueous media. Methyltetrazine-PEG4-STP Ester undergoes hydrolysis in aqueous media at much slower rate compared to Methyltetrazine-Sulfo NHS Ester allowing for more efficient labeling of biomolecules in aqueous media. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary TCO-containing molecules. The aqueous solubility of this reagent is substantially enhanced by a hydrophilic polyethylene glycol (PEG) spacer arm.

4-Sulfo-2,3,5,6-tetrafluorophenyl (STP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, STP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.

Specifications

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Methyltetrazine-activated biotinylation reagent that reacts with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 M^-1 s^-1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

Specifications

The post Biotin-PEG4-Methyltetrazine appeared first on VectorLabs.

Methyltetrazine-activated PEGylation reagent that reacts with TCO-containing compounds via Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures.

Specifications

The post Methyltetrazine-mPEG, 10 kDa appeared first on VectorLabs.

Methyltetrazine-activated PEGylation reagent that reacts with TCO-containing compounds via Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures.

Specifications

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Methyltetrazine-PEG4-Azide is a heterobifunctional, alkyne-to-TCO crosslinker with azide and methyltetrazine groups connected through a PEG4 spacer. Often used to convert a readily available terminal alkyne groups into methyltetrazine moiety through CuAAC reaction.

Specifications

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Methyltetrazine-PEG4-Alkyne is a heterobifunctional, azide-to-TCO crosslinker with terminal alkyne and methyltetrazine groups connected through a PEG4 spacer. Often used to convert a readily available azide groups into methyltetrazine moiety through CuAAC reaction.

Specifications

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The post Methyltetrazine DBCO appeared first on VectorLabs.

Methyltetrazine-PEG4-Maleimide is a sulfhydryl-reactive reagent that contains a 4-unit ethylene glycol in its spacer arm for increased water-solubility. It enables simple and efficient incorporation of a methyltetrazine moiety onto antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. The hydrophilic polyethylene glycol (PEG) spacer arm improves solubility in aqueous buffers.

Specifications

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A building block used for derivatization of carboxylic acids or activated esters with methyltetrazine moiety. The stability of Methyltetrazine-PEG4-Amine is substantially improved compared to hydrogen substituted tetrazines. The aqueous solubility of this reagent is substantially enhanced by a hydrophilic polyethylene glycol (PEG) spacer arm. This reagent is supplied as HCl salt for improved stability and easy handling

Specifications

The post Methyltetrazine-PEG4-Amine appeared first on VectorLabs.

A building block used for derivatization of carboxylic acids or activated esters with tetrazine moiety. Tetrazine Amine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctene (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. This reagent is supplied as HCl salt for improved stability and easy handling.

Specifications

The post Tetrazine Amine appeared first on VectorLabs.