Labeling Kits – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:06:23 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Labeling Kits – VectorLabs https://staging.vectorlabs.com 32 32 Click-&-Go™ Lys-to-Lys Protein-Protein Conjugation Kit https://staging.vectorlabs.com/products/click-go-lys-to-lys-protein-protein-conjugation-kit/ Tue, 19 Sep 2023 19:54:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=22258 The Lys-to-Lys Protein-Protein Conjugation Kit (Cat. # 1008) provides a sufficient amount of reagents to perform two protein-protein conjugation reactions. Any two lysine containing proteins (100-500 µg) in a volume of 100 µL (1-5 mg/mL) can be efficiently conjugated in less than 3 hours, start-to-finish.

The Lys-to-Lys Protein-Protein Conjugation kit provides all of the necessary reagents to perform two protein-protein conjugation reactions. Conjugates are formed by the very efficient, catalyst-free bio-orthogonal ligation reaction between trans-cyclooctene (TCO) and tetrazine (Tz) functional groups. In the protocol provided, two proteins (or any other amine-containing biopolymers) are labeled separately with TCO and Tetrazine reagents. The labeled molecules are then mixed for 60 minutes in any suitable buffer to form a conjugate.

The extremely fast kinetics of the TCO/Tz ligation reaction enables rapid conjugation (30-60 min) of lysine containing proteins to each other at low concentrations (e.g. 5-10 µM), with >99% conversion of the limiting protein in mild buffered media (e.g. PBS pH 7.5). Another benefit is the long-term stability of TCO and Tz functional groups on modified proteins stored in aqueous buffered media (e.g. maintaining > 90% reactivity after 1 month at 4oC, pH 7.5). This stability allows for worry-free conjugation results without the sensitive timing of reactions associated with classical thiol/maleimide chemistry.

Specifications

Unit Size 1 kit
Number of Reactions 2
Storage Conditions 4C
Shipping Conditions Ambient temperature

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Description

The Lys-to-Lys Protein-Protein Conjugation Kit (Cat. # 1008) provides a sufficient amount of reagents to perform two protein-protein conjugation reactions. Any two lysine containing proteins (100-500 µg) in a volume of 100 µL (1-5 mg/mL) can be efficiently conjugated in less than 3 hours, start-to-finish.

The Lys-to-Lys Protein-Protein Conjugation kit provides all of the necessary reagents to perform two protein-protein conjugation reactions. Conjugates are formed by the very efficient, catalyst-free bio-orthogonal ligation reaction between trans-cyclooctene (TCO) and tetrazine (Tz) functional groups. In the protocol provided, two proteins (or any other amine-containing biopolymers) are labeled separately with TCO and Tetrazine reagents. The labeled molecules are then mixed for 60 minutes in any suitable buffer to form a conjugate.

The extremely fast kinetics of the TCO/Tz ligation reaction enables rapid conjugation (30-60 min) of lysine containing proteins to each other at low concentrations (e.g. 5-10 µM), with >99% conversion of the limiting protein in mild buffered media (e.g. PBS pH 7.5). Another benefit is the long-term stability of TCO and Tz functional groups on modified proteins stored in aqueous buffered media (e.g. maintaining > 90% reactivity after 1 month at 4oC, pH 7.5). This stability allows for worry-free conjugation results without the sensitive timing of reactions associated with classical thiol/maleimide chemistry.

1008 schamtic workflow
Figure 1. Schematic representation of protein-protein conjugation.

Specifications

Unit Size1 kit

Number of Reactions

2
Storage Conditions4C
Shipping ConditionsAmbient temperature

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

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]]> Click-&-Go® Cell Reaction Buffer Kit https://staging.vectorlabs.com/products/click-go-cell-reaction-buffer-kit/ Tue, 19 Sep 2023 19:23:35 +0000 https://staging.vectorlabs.com/?post_type=product&p=21861 Description

Click-&-Go™ Cell Reaction Buffer Kit provides researchers everything required to perform the click reaction on cells tagged with an azide or alkyne and with the corresponding click detection reagent for subsequent downstream analysis by flow cytometry, fluorescence microscopy, or high content screening (HCS).

Staining with additional detection reagents can performed prior or after the click reaction. Each kit includes sufficient material to perform 50 reactions based on 0.5 mL volume.

Specifications

Unit Size 1 kit
Number of Reactions
 50
Storage Conditions 4C
Shipping Conditions Ambient temperature

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Click-&-Go® Cell Reaction Buffer Kit provides researchers everything required to perform the click reaction on cells tagged with an azide or alkyne and with the corresponding click detection reagent for subsequent downstream analysis by flow cytometry, fluorescence microscopy, or high content screening (HCS). Staining with additional detection reagents can performed prior or after the click reaction. Each kit includes sufficient material to perform 50 reactions based on 0.5 mL volume.

Description

Click-&-Go™ Cell Reaction Buffer Kit provides researchers everything required to perform the click reaction on cells tagged with an azide or alkyne and with the corresponding click detection reagent for subsequent downstream analysis by flow cytometry, fluorescence microscopy, or high content screening (HCS).

Click reaction 1

Staining with additional detection reagents can performed prior or after the click reaction. Each kit includes sufficient material to perform 50 reactions based on 0.5 mL volume.

Specifications

Unit Size1 kit
Number of Reactions
50
Storage Conditions4C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Costello, A., et al. (2021). Directed evolution of rRNA improves translation kinetics and recombinant protein yield. Nat Commun., 12 (1), 5638. [PubMed]

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]]> Click-&-Go® Click Chemistry Reaction Buffer Kit https://staging.vectorlabs.com/products/click-go-click-chemistry-reaction-buffer-kit/ Tue, 19 Sep 2023 19:23:32 +0000 https://staging.vectorlabs.com/?post_type=product&p=21852 Description

Click Chemistry Reaction Buffer Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click detection reagent with all the necessary reagents to perform an up to 25 efficient copper-catalyzed click reactions for subsequent analysis by gel electrophoresis, western blot or mass spectrometry. The kit utilizes THPTA as a copper chelating reagent to stabilize a catalytically active Cu(I) to accelerate the rate of CuAAC. The use of copper-chelating ligand also minimizes amount of free copper in solution, thus limiting the generation of reactive oxygen species (ROS) and protecting against undesired oxidative damage side reactions to proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and other biomolecules.

Specifications

Unit Size 1 Kit
Number of Reactions
25
Storage Condition 4 C
Shipping Conditions
Ambient temperature

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Description

Click-&-Go® Click Chemistry Reaction Buffer Kit provides researchers who have biomolecules labeled with an azide or alkyne and the corresponding click detection reagent with all the necessary reagents to perform a copper-catalyzed ligation reaction. Sufficient materials provided to perform up to 25 copper-catalyzed click reactions for subsequent analysis by gel electrophoresis, western blot or mass spectrometry.

Click reaction

The kit utilizes THPTA as a copper chelating reagent to stabilize a catalytically active Cu(I) to accelerate the rate of CuAAC. The use of copper-chelating ligand also minimizes amount of free copper in solution, thus limiting the generation of reactive oxygen species (ROS) and protecting against undesired oxidative damage side reactions to proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and other biomolecules.

Sufficient reagents are provided for 25 labeling reactions containing 50 uL protein solution (1-5 mg/mL).

Specifications

Unit Size1 Kit
Number of Reactions
25
Storage Condition4 C
Shipping Conditions
Ambient temperature

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

Selected References

  1. Lee, M.T., et al. (2020). Dietary sphinganine is selectively assimilated by members of themammaliangut microbiome. J Lipid Res . [PubMed]
 

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]]> Click-&-Go® Protein Reaction Buffer Kit https://staging.vectorlabs.com/products/click-go-protein-reaction-buffer-kit/ Tue, 19 Sep 2023 17:16:11 +0000 https://staging.vectorlabs.com/?post_type=product&p=20648 Description

Click-&-Go Click Chemistry Reaction Buffer Kit provides researchers who have biomolecules labeled with an azide or alkyne and the corresponding click detection reagent with all the necessary reagents to perform an up to 25 copper-catalyzed click reactions for subsequent analysis by gel electrophoresis, western blot or mass spectrometry.

The kit utilizes a copper chelating reagent to stabilize a catalytically active Cu(I) to accelerate the rate of CuAAC. The use of copper-chelating ligand also minimizes amount of free copper in solution, thus limiting the generation of reactive oxygen species (ROS) and protecting against undesired oxidative damage side reactions to proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and other biomolecules.

Specifications

Unit Size 1 kit
Number of Reactions
 25
Storage Conditions 4C
Shipping Conditions Ambient temperature

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Click-&-Go® Protein Reaction Buffer Kit provides researchers everything required to perform the click reaction on azide or alkyne tagged proteins with the corresponding click detection reagent for subsequent downstream analysis. Each kit includes sufficient material to perform 25 reactions for subsequent analysis by gel electrophoresis, western blot or mass spectrometry.

Description

Click-&-Go Click Chemistry Reaction Buffer Kit provides researchers who have biomolecules labeled with an azide or alkyne and the corresponding click detection reagent with all the necessary reagents to perform an up to 25 copper-catalyzed click reactions for subsequent analysis by gel electrophoresis, western blot or mass spectrometry.

1262 scheme 01

The kit utilizes a copper chelating reagent to stabilize a catalytically active Cu(I) to accelerate the rate of CuAAC. The use of copper-chelating ligand also minimizes amount of free copper in solution, thus limiting the generation of reactive oxygen species (ROS) and protecting against undesired oxidative damage side reactions to proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and other biomolecules.

Specifications

Unit Size1 kit
Number of Reactions
25
Storage Conditions4C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Bruna, R. E., et al. (2021). Limitation of phosphate assimilation maintains cytoplasmic magnesium homeostasis. Proc Natl Acad Sci U S A., 118 (11), e2021370118. [PubMed]
  2. Kwong, J. Q., et al. (2021). Mitochondrial functional resilience after TFAM ablation in the adult heart. Am J Physiol Cell Physiol., 320 (6), C929-C942. [PubMed]
  3. Coates, H. W. et al. (2021). The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form. J Biol Chem., Online ahead of print. [PubMed]

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