DBCO Reagents – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:21:59 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png DBCO Reagents – VectorLabs https://staging.vectorlabs.com 32 32 DBCO-TFP ester https://staging.vectorlabs.com/products/dbco-tfp-ester/ Sat, 24 Feb 2024 02:18:09 +0000 https://staging.vectorlabs.com/?post_type=product&p=47562 DBCO-TFP ester, product number QBD-11815, is the 2,3,5,6-tetrafluorophenyl (TFP) ester of an aza-dibenzocyclooctyne (DBCO) product used for strain-promoted azide-alkyne cycloaddition (SPAAC, also known as “”copper free click chemistry””). The TFP ester reacts selectively and efficiently with amines to form amide bonds. TFP esters are preferred over N-hydroxysuccinimide (NHS) esters because they are more stable to hydrolysis and react more efficiently with amines than NHS esters. The DBCO moiety reacts selectively with azides via SPAAC to form stable triazole linkages. Insoluble in water, DBCO-TFP ester is slightly soluble to sparingly soluble in most organic solvents. Vector Laboratories recommends dimethylsulfoxide (DMSO), N,N’-dimethylformamide (DMF), or N,N’-dimethylacetamide (DMAC) as solvents for this product. DBCO-TFP ester is acid sensitive. It should not be subjected to pH <5 during reaction or workup.

The published discovery in 2004 of SPAAC, also known as “”copper free click chemistry,”” by Carolyn Bertozzi and coworkers improved the click chemistry toolbox. Toxic copper catalysts were eliminated, making the reaction truly bioorthogonal. By removing the copper(I) catalyst from the reaction, living cells can be labeled with reagents designed for SPAAC. Since the original publication, multiple improvements in strained cyclooctyne reagents resulted in the development of DBCO as a nearly perfect azide partner for SPAAC.

To use DBCO-TFP ester in aqueous media or in cells, it should be conjugated to a water-soluble dPEG® product. Vector Laboratories offers numerous products for this purpose, including amino alcohols and amino acids. We also offer many ready-to-use DBCO-functionalized dPEG® products.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 467.41; single compound
Chemical formula C₂₆H₁₇F₄NO₃
CAS N/A
Purity > 98%
Spacers N/A
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Chloroform, DMSO, DMAC, or DMF.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post DBCO-TFP ester appeared first on VectorLabs.

]]>

Description

DBCO-TFP ester, product number QBD-11815, is the 2,3,5,6-tetrafluorophenyl (TFP) ester of an aza-dibenzocyclooctyne (DBCO) product used for strain-promoted azide-alkyne cycloaddition (SPAAC, also known as “”copper free click chemistry””). The TFP ester reacts selectively and efficiently with amines to form amide bonds. TFP esters are preferred over N-hydroxysuccinimide (NHS) esters because they are more stable to hydrolysis and react more efficiently with amines than NHS esters. The DBCO moiety reacts selectively with azides via SPAAC to form stable triazole linkages. Insoluble in water, DBCO-TFP ester is slightly soluble to sparingly soluble in most organic solvents. Vector Laboratories recommends dimethylsulfoxide (DMSO), N,N’-dimethylformamide (DMF), or N,N’-dimethylacetamide (DMAC) as solvents for this product. DBCO-TFP ester is acid sensitive. It should not be subjected to pH <5 during reaction or workup.

The published discovery in 2004 of SPAAC, also known as “”copper free click chemistry,”” by Carolyn Bertozzi and coworkers improved the click chemistry toolbox. Toxic copper catalysts were eliminated, making the reaction truly bioorthogonal. By removing the copper(I) catalyst from the reaction, living cells can be labeled with reagents designed for SPAAC. Since the original publication, multiple improvements in strained cyclooctyne reagents resulted in the development of DBCO as a nearly perfect azide partner for SPAAC.

To use DBCO-TFP ester in aqueous media or in cells, it should be conjugated to a water-soluble dPEG® product. Vector Laboratories offers numerous products for this purpose, including amino alcohols and amino acids. We also offer many ready-to-use DBCO-functionalized dPEG® products.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight467.41; single compound
Chemical formulaC₂₆H₁₇F₄NO₃
CASN/A
Purity> 98%
SpacersN/A
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Chloroform, DMSO, DMAC, or DMF.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post DBCO-TFP ester appeared first on VectorLabs.

]]> DBCO-acid https://staging.vectorlabs.com/products/dbco-acid-2/ Sat, 24 Feb 2024 02:18:08 +0000 https://staging.vectorlabs.com/?post_type=product&p=39853 DBCO-acid, product number QBD-11814, is an aza-dibenzocyclooctyne (DBCO) product used for strain-promoted azide-alkyne cycloaddition (SPAAC, also known as ""copper free click chemistry""). The DBCO moiety reacts selectively with azides via SPAAC to form stable triazole linkages. Esterification of the terminal carboxylic acid with N-hydroxysuccinimide (NHS) or 2,3,5,6-tetrafluorophenol (TFP) allows for reaction with a primary amine. Insoluble in water, DBCO-acid is slightly soluble to sparingly soluble in most organic solvents. Vector Laboratories recommends dimethylsulfoxide (DMSO), N,N'-dimethylformamide (DMF), or N,N'-dimethylacetamide (DMAC) as solvents for this product. DBCO-acid is relatively insensitive to bases and can be used in the presence of aqueous bases. This product is acid sensitive and should not be subjected to pH <5 during reaction or workup.

The published discovery in 2004 of SPAAC, also known as ""copper free click chemistry,"" by Carolyn Bertozzi and coworkers improved the click chemistry toolbox. Toxic copper catalysts were eliminated, making the reaction truly bioorthogonal. By removing the copper(I) catalyst from the reaction, living cells can be labeled with reagents designed for SPAAC. Since the original publication, multiple improvements in strained cyclooctyne reagents resulted in the development of DBCO as a nearly perfect azide partner for SPAAC.

To use DBCO-acid in aqueous media or in cells, it should be conjugated to a water-soluble dPEG® product. Vector Laboratories offers numerous products for this purpose, including amino alcohols and amino acids. We also offer many DBCO-based dPEG® products already prepared for use.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 319.35; single compound
Chemical formula C₂₀H₁₇NO₃
CAS N/A
Purity > 98%
Spacers N/A
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) DMAC, DMSO, or DMF
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post DBCO-acid appeared first on VectorLabs.

]]>

Description

DBCO-acid, product number QBD-11814, is an aza-dibenzocyclooctyne (DBCO) product used for strain-promoted azide-alkyne cycloaddition (SPAAC, also known as “”copper free click chemistry””). The DBCO moiety reacts selectively with azides via SPAAC to form stable triazole linkages. Esterification of the terminal carboxylic acid with N-hydroxysuccinimide (NHS) or 2,3,5,6-tetrafluorophenol (TFP) allows for reaction with a primary amine. Insoluble in water, DBCO-acid is slightly soluble to sparingly soluble in most organic solvents. Vector Laboratories recommends dimethylsulfoxide (DMSO), N,N’-dimethylformamide (DMF), or N,N’-dimethylacetamide (DMAC) as solvents for this product. DBCO-acid is relatively insensitive to bases and can be used in the presence of aqueous bases. This product is acid sensitive and should not be subjected to pH <5 during reaction or workup.

The published discovery in 2004 of SPAAC, also known as “”copper free click chemistry,”” by Carolyn Bertozzi and coworkers improved the click chemistry toolbox. Toxic copper catalysts were eliminated, making the reaction truly bioorthogonal. By removing the copper(I) catalyst from the reaction, living cells can be labeled with reagents designed for SPAAC. Since the original publication, multiple improvements in strained cyclooctyne reagents resulted in the development of DBCO as a nearly perfect azide partner for SPAAC.

To use DBCO-acid in aqueous media or in cells, it should be conjugated to a water-soluble dPEG® product. Vector Laboratories offers numerous products for this purpose, including amino alcohols and amino acids. We also offer many DBCO-based dPEG® products already prepared for use.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight319.35; single compound
Chemical formulaC₂₀H₁₇NO₃
CASN/A
Purity> 98%
SpacersN/A
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)DMAC, DMSO, or DMF
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post DBCO-acid appeared first on VectorLabs.

]]> DBCO-amine https://staging.vectorlabs.com/products/dbco-amine-2/ Sat, 24 Feb 2024 02:17:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=39828 DBCO-amine, product number QBD-11750, is an aza-dibenzocyclooctyne (DBCO) product used for strain-promoted azide-alkyne cycloaddition (SPAAC, also known as ""copper free click chemistry""). The DBCO moiety reacts selectively with azides via SPAAC to form stable triazole linkages. The amine terminus of the molecule reacts with carboxylic acids and their active esters to form stable amide bonds. Insoluble in water, DBCO-amine is slightly soluble to sparingly soluble in most organic solvents. Vector Laboratories recommends dimethylsulfoxide (DMSO), N,N'-dimethylformamide (DMF), or N,N'-dimethylacetamide (DMAC) as solvents for this product. DBCO-amine is relatively insensitive to bases and can be used in the presence of aqueous bases. This product is acid sensitive. It should not be subjected to pH <5 during reaction or workup. The product should always be stored at -20°C when not in use.

The published discovery in 2004 of SPAAC, also known as ""copper free click chemistry,"" by Carolyn Bertozzi and coworkers improved the click chemistry toolbox. Toxic copper catalysts were eliminated, making the reaction truly bioorthogonal. By removing the copper(I) catalyst from the reaction, living cells can be labeled with reagents designed for SPAAC. Since the original publication, multiple improvements in strained cyclooctyne reagents resulted in the development of DBCO as a nearly perfect azide partner for SPAAC.

To use DBCO-amine effectively in aqueous media or in cells, it should be conjugated to a water-soluble dPEG® product. Vector Laboratories offers numerous products for this purpose, including hydroxy acids (and their active esters), and protected amino acids. We also offer many DBCO-based dPEG® products already prepared for use.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 276.33; single compound
Chemical formula C₁₈H₁₆N₂O
CAS N/A
Purity > 97%
Spacers N/A
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, Acetone, DMSO, or Acetonitrile.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post DBCO-amine appeared first on VectorLabs.

]]>

Description

DBCO-amine, product number QBD-11750, is an aza-dibenzocyclooctyne (DBCO) product used for strain-promoted azide-alkyne cycloaddition (SPAAC, also known as “”copper free click chemistry””). The DBCO moiety reacts selectively with azides via SPAAC to form stable triazole linkages. The amine terminus of the molecule reacts with carboxylic acids and their active esters to form stable amide bonds. Insoluble in water, DBCO-amine is slightly soluble to sparingly soluble in most organic solvents. Vector Laboratories recommends dimethylsulfoxide (DMSO), N,N’-dimethylformamide (DMF), or N,N’-dimethylacetamide (DMAC) as solvents for this product. DBCO-amine is relatively insensitive to bases and can be used in the presence of aqueous bases. This product is acid sensitive. It should not be subjected to pH <5 during reaction or workup. The product should always be stored at -20°C when not in use.

The published discovery in 2004 of SPAAC, also known as “”copper free click chemistry,”” by Carolyn Bertozzi and coworkers improved the click chemistry toolbox. Toxic copper catalysts were eliminated, making the reaction truly bioorthogonal. By removing the copper(I) catalyst from the reaction, living cells can be labeled with reagents designed for SPAAC. Since the original publication, multiple improvements in strained cyclooctyne reagents resulted in the development of DBCO as a nearly perfect azide partner for SPAAC.

To use DBCO-amine effectively in aqueous media or in cells, it should be conjugated to a water-soluble dPEG® product. Vector Laboratories offers numerous products for this purpose, including hydroxy acids (and their active esters), and protected amino acids. We also offer many DBCO-based dPEG® products already prepared for use.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight276.33; single compound
Chemical formulaC₁₈H₁₆N₂O
CASN/A
Purity> 97%
SpacersN/A
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, Acetone, DMSO, or Acetonitrile.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post DBCO-amine appeared first on VectorLabs.

]]> Bis-dPEG®₁₁-DBCO https://staging.vectorlabs.com/products/bis-dpeg11-dbco/ Sat, 24 Feb 2024 02:16:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=39481 Bis-dPEG®11-DBCO, product number QBD-11372, is a homobifunctional click chemistry crosslinker consisting of two dibenzylcyclooctyne (DBCO) groups separated by a medium-length (39 atoms, 44.2 Å), single molecular weight, discrete polyethylene glycol (dPEG®) spacer. The DBCO groups react with azide groups via the bioorthogonal click chemistry reaction known as strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. This product is particularly useful for click chemistry reactions where the presence or use of copper(I) [Cu(I)] is undesirable. Any two molecules containing accessible azide groups can be crosslinked with this compound. Applications for Bis-dPEG®11-DBCO include crosslinking biomolecules that have been modified to include azide groups and construction of complex supramolecular structures.

Specifications

Unit Size 25 mg, 100 mg, 500 mg
Molecular Weight 1147.35; single compound
Chemical formula C₆₄H₈₂N₄O₁₅
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 39 atoms and 44.2 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, DMAC, DMF, or DMSO
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₁₁-DBCO appeared first on VectorLabs.

]]>

Description

Bis-dPEG®11-DBCO, product number QBD-11372, is a homobifunctional click chemistry crosslinker consisting of two dibenzylcyclooctyne (DBCO) groups separated by a medium-length (39 atoms, 44.2 Å), single molecular weight, discrete polyethylene glycol (dPEG®) spacer. The DBCO groups react with azide groups via the bioorthogonal click chemistry reaction known as strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. This product is particularly useful for click chemistry reactions where the presence or use of copper(I) [Cu(I)] is undesirable. Any two molecules containing accessible azide groups can be crosslinked with this compound. Applications for Bis-dPEG®11-DBCO include crosslinking biomolecules that have been modified to include azide groups and construction of complex supramolecular structures.

Specifications

Unit Size25 mg, 100 mg, 500 mg
Molecular Weight1147.35; single compound
Chemical formulaC₆₄H₈₂N₄O₁₅
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 39 atoms and 44.2 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, DMAC, DMF, or DMSO
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post Bis-dPEG®₁₁-DBCO appeared first on VectorLabs.

]]> Tetra(-dPEG®₁₁-DBCO)pentaerythritol https://staging.vectorlabs.com/products/tetra-dpeg11-dbcopentaerythritol/ Sat, 24 Feb 2024 02:16:46 +0000 https://staging.vectorlabs.com/?post_type=product&p=39473 Tetra(-dPEG®11-DBCO)pentaerythritol, product number QBD-11371, is a four-armed, discrete polyethylene glycol (dPEG®) crosslinker built around a pentaerythritol core. Each dPEG®11 spacer arm is 43 atoms (47.4 Å) long and terminates with a dibenzocyclooctyne (DBCO) functional group. The DBCO moiety conjugates to an azide-functionalized molecule or surface via strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. This product is useful for supramolecular construction (e.g., dendrimers) and for efficiently crosslinking azide-functionalized proteins or peptides.

This compound showcases the advantages of dPEG® products. Conventional polyethylene glycol (PEG) products are dispersed polymers. These polymers consist of a complex mixture of different chain lengths and molecular weights that make exact analysis and characterization quite challenging.

Vector Laboratories' dPEG® products contain precisely defined, single molecular weight, discrete-length PEG chains (i.e., they are monodispersed PEG products). Tetra(-dPEG®11-DBCO)pentaerythritol would be challenging to characterize if made with conventional PEG reagents because of polymer dispersity. The synthesis of a product like Tetra(-dPEG®11-DBCO)pentaerythritol using standard PEG reagents creates a complicated assortment of different products. However, using dPEG® reagents, this product can be synthesized and characterized accurately.

Specifications

Unit Size 25 mg, 100 mg
Molecular Weight 3736.451; single compound
Chemical formula C₁₉₃H₂₂₈N₁₂O₆₀
CAS N/A
Purity > 97%
Spacers dPEG® Spacer data for each arm is 43 atoms and 47.4 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, DMAC, DMF, or DMSO
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Tetra(-dPEG®₁₁-DBCO)pentaerythritol appeared first on VectorLabs.

]]>

Description

Tetra(-dPEG®11-DBCO)pentaerythritol, product number QBD-11371, is a four-armed, discrete polyethylene glycol (dPEG®) crosslinker built around a pentaerythritol core. Each dPEG®11 spacer arm is 43 atoms (47.4 Å) long and terminates with a dibenzocyclooctyne (DBCO) functional group. The DBCO moiety conjugates to an azide-functionalized molecule or surface via strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. This product is useful for supramolecular construction (e.g., dendrimers) and for efficiently crosslinking azide-functionalized proteins or peptides.

This compound showcases the advantages of dPEG® products. Conventional polyethylene glycol (PEG) products are dispersed polymers. These polymers consist of a complex mixture of different chain lengths and molecular weights that make exact analysis and characterization quite challenging.

Vector Laboratories’ dPEG® products contain precisely defined, single molecular weight, discrete-length PEG chains (i.e., they are monodispersed PEG products). Tetra(-dPEG®11-DBCO)pentaerythritol would be challenging to characterize if made with conventional PEG reagents because of polymer dispersity. The synthesis of a product like Tetra(-dPEG®11-DBCO)pentaerythritol using standard PEG reagents creates a complicated assortment of different products. However, using dPEG® reagents, this product can be synthesized and characterized accurately.

Specifications

Unit Size25 mg, 100 mg
Molecular Weight3736.451; single compound
Chemical formulaC₁₉₃H₂₂₈N₁₂O₆₀
CASN/A
Purity> 97%
SpacersdPEG® Spacer data for each arm is 43 atoms and 47.4 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, DMAC, DMF, or DMSO
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post Tetra(-dPEG®₁₁-DBCO)pentaerythritol appeared first on VectorLabs.

]]> DBCO-dPEG®₂₄-TFP ester https://staging.vectorlabs.com/products/dbco-dpeg24-tfp-ester/ Sat, 24 Feb 2024 02:16:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=39464 DBCO-dPEG®24-TFP ester, product number QBD-11370, is a heterobifunctional, bioorthogonal crosslinker designed to join azides and amines across a long (76 atoms, 88.8 Å), flexible, hydrophilic, single molecular weight, discrete PEG (dPEG®) spacer. The tetrafluorophenyl (TFP) ester reacts specifically with free amines to form amide bonds; the optimum reaction pH is 7.5 - 8.0. The dibenzylcyclooctyne (DBCO) moiety selectively forms triazole linkages with azides via a click chemistry reaction known as strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. The amphiphilic dPEG® linker increases conjugates' hydrodynamic volumes, and it can be used to create space in sterically crowded supramolecular constructs.

Specifications

Unit Size 25 mg, 100 mg, 500 mg
Molecular Weight 1595.75; single compound
Chemical formula C₇₇H₁₁₈F₄N₂O₂₈
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 76 atoms and 88.8 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, DMSO, DMAC, DMF, or Acetonitrile.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post DBCO-dPEG®₂₄-TFP ester appeared first on VectorLabs.

]]>

Description

DBCO-dPEG®24-TFP ester, product number QBD-11370, is a heterobifunctional, bioorthogonal crosslinker designed to join azides and amines across a long (76 atoms, 88.8 Å), flexible, hydrophilic, single molecular weight, discrete PEG (dPEG®) spacer. The tetrafluorophenyl (TFP) ester reacts specifically with free amines to form amide bonds; the optimum reaction pH is 7.5 – 8.0. The dibenzylcyclooctyne (DBCO) moiety selectively forms triazole linkages with azides via a click chemistry reaction known as strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. The amphiphilic dPEG® linker increases conjugates’ hydrodynamic volumes, and it can be used to create space in sterically crowded supramolecular constructs.

Specifications

Unit Size25 mg, 100 mg, 500 mg
Molecular Weight1595.75; single compound
Chemical formulaC₇₇H₁₁₈F₄N₂O₂₈
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 76 atoms and 88.8 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, DMSO, DMAC, DMF, or Acetonitrile.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post DBCO-dPEG®₂₄-TFP ester appeared first on VectorLabs.

]]> DBCO-dPEG®₁₂-TFP ester https://staging.vectorlabs.com/products/dbco-dpeg12-tfp-ester/ Sat, 24 Feb 2024 02:16:44 +0000 https://staging.vectorlabs.com/?post_type=product&p=39455 DBCO-dPEG®12-TFP ester, product number QBD-11366, is a heterobifunctional, bioorthogonal crosslinker designed to join azides and amines across a medium-length (40 atoms, 46.3 Å), flexible, hydrophilic, single molecular weight, discrete PEG (dPEG®) spacer. The tetrafluorophenyl (TFP) ester reacts specifically with free amines to form amide bonds; the optimum reaction pH is 7.5 - 8.0. The dibenzylcyclooctyne (DBCO) moiety selectively forms triazole linkages with azides via a click chemistry reaction known as strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. The amphiphilic dPEG® linker increases conjugates' hydrodynamic volumes, and it can be used to create space in sterically crowded supramolecular constructs.

Specifications

Unit Size 25 mg, 100 mg, 500 mg
Molecular Weight 1067.12; single compound
Chemical formula C₅₃H₇₀F₄N₂O₁₆
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 40 atoms and 46.3 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, DMSO, DMAC, Methanol, DMF, or Acetonitrile.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post DBCO-dPEG®₁₂-TFP ester appeared first on VectorLabs.

]]>

Description

DBCO-dPEG®12-TFP ester, product number QBD-11366, is a heterobifunctional, bioorthogonal crosslinker designed to join azides and amines across a medium-length (40 atoms, 46.3 Å), flexible, hydrophilic, single molecular weight, discrete PEG (dPEG®) spacer. The tetrafluorophenyl (TFP) ester reacts specifically with free amines to form amide bonds; the optimum reaction pH is 7.5 – 8.0. The dibenzylcyclooctyne (DBCO) moiety selectively forms triazole linkages with azides via a click chemistry reaction known as strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. The amphiphilic dPEG® linker increases conjugates’ hydrodynamic volumes, and it can be used to create space in sterically crowded supramolecular constructs.

Specifications

Unit Size25 mg, 100 mg, 500 mg
Molecular Weight1067.12; single compound
Chemical formulaC₅₃H₇₀F₄N₂O₁₆
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 40 atoms and 46.3 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, DMSO, DMAC, Methanol, DMF, or Acetonitrile.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post DBCO-dPEG®₁₂-TFP ester appeared first on VectorLabs.

]]> DBCO-dPEG®₄-TFP ester https://staging.vectorlabs.com/products/dbco-dpeg4-tfp-ester/ Sat, 24 Feb 2024 02:16:43 +0000 https://staging.vectorlabs.com/?post_type=product&p=39440 DBCO-dPEG®4-TFP ester, product number QBD-11362, is a heterobifunctional, bioorthogonal crosslinker designed to join azides and amines across a short (16 atoms, 17.9 Å), flexible, hydrophilic, single molecular weight, discrete PEG (dPEG®) spacer. The tetrafluorophenyl (TFP) ester reacts specifically with free amines to form amide bonds; the optimum reaction pH is 7.5 - 8.0. The dibenzylcyclooctyne (DBCO) moiety selectively forms triazole linkages with azides via a click chemistry reaction known as strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. The amphiphilic dPEG® linker increases conjugates' hydrodynamic volumes, and it can be used to create space in sterically crowded supramolecular constructs.

Specifications

Unit Size 25 mg, 100 mg, 500 mg
Molecular Weight 714.70; single compound
Chemical formula C₃₇H₃₈F₄N₂O₈
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 16 atoms and 17.9 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, DMSO, DMAC, Methanol, or Acetonitrile.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post DBCO-dPEG®₄-TFP ester appeared first on VectorLabs.

]]>

Description

DBCO-dPEG®4-TFP ester, product number QBD-11362, is a heterobifunctional, bioorthogonal crosslinker designed to join azides and amines across a short (16 atoms, 17.9 Å), flexible, hydrophilic, single molecular weight, discrete PEG (dPEG®) spacer. The tetrafluorophenyl (TFP) ester reacts specifically with free amines to form amide bonds; the optimum reaction pH is 7.5 – 8.0. The dibenzylcyclooctyne (DBCO) moiety selectively forms triazole linkages with azides via a click chemistry reaction known as strain-promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry. The amphiphilic dPEG® linker increases conjugates’ hydrodynamic volumes, and it can be used to create space in sterically crowded supramolecular constructs.

Specifications

Unit Size25 mg, 100 mg, 500 mg
Molecular Weight714.70; single compound
Chemical formulaC₃₇H₃₈F₄N₂O₈
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 16 atoms and 17.9 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, DMSO, DMAC, Methanol, or Acetonitrile.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post DBCO-dPEG®₄-TFP ester appeared first on VectorLabs.

]]> Bromoacetamido-dPEG®₂₄-amido-DBCO https://staging.vectorlabs.com/products/bromoacetamido-dpeg24-amido-dbco/ Sat, 24 Feb 2024 02:16:32 +0000 https://staging.vectorlabs.com/?post_type=product&p=39332 Bromoacetamido-dPEG®24-amido-DBCO, product number 11224, combines a thiol-reactive bromoacetyl group with dibenzylcyclooctyne (DBCO, also known as DIBAC), a copper-free click chemistry reactive group. The two reactive groups link via a long (76 atoms, 90.0 Å), flexible, single molecular weight, discrete polyethylene glycol (dPEG®) linker. The bromoacetyl group reacts chemoselectively with thiols at pH ≥ 8.0. This differs from the maleimide group, which reacts chemoselectively with sulfhydryls within the range of pH 6.5 – 7.5. The DBCO moiety is a strained cyclooctyne ring flanked by two benzyl rings on either side. DBCO was designed for strain promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry.

Specifications

Unit Size 25 mg, 100 mg, 500 mg
Molecular Weight 1525.60; single compound
Chemical formula C₇₁H₁₁₈BrN₃O₂₇
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 76 atoms and 90.0 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bromoacetamido-dPEG®₂₄-amido-DBCO appeared first on VectorLabs.

]]>

Description

Bromoacetamido-dPEG®24-amido-DBCO, product number QBD-11224, combines a thiol-reactive bromoacetyl group with dibenzylcyclooctyne (DBCO, also known as DIBAC), a copper-free click chemistry reactive group. The two reactive groups link via a long (76 atoms, 90.0 Å), flexible, single molecular weight, discrete polyethylene glycol (dPEG®) linker. The bromoacetyl group reacts chemoselectively with thiols at pH ≥ 8.0. This differs from the maleimide group, which reacts chemoselectively with sulfhydryls within the range of pH 6.5 – 7.5. The DBCO moiety is a strained cyclooctyne ring flanked by two benzyl rings on either side. DBCO was designed for strain promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry.

Specifications

Unit Size25 mg, 100 mg, 500 mg
Molecular Weight1525.60; single compound
Chemical formulaC₇₁H₁₁₈BrN₃O₂₇
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 76 atoms and 90.0 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post Bromoacetamido-dPEG®₂₄-amido-DBCO appeared first on VectorLabs.

]]> Bromoacetamido-dPEG®₁₂-amido-DBCO https://staging.vectorlabs.com/products/bromoacetamido-dpeg12-amido-dbco/ Sat, 24 Feb 2024 02:16:29 +0000 https://staging.vectorlabs.com/?post_type=product&p=39323 Bromoacetamido-dPEG®12-amido-DBCO, product number 11223, combines a thiol-reactive bromoacetyl group with dibenzylcyclooctyne (DBCO, also known as DIBAC), a copper-free click chemistry reactive group. The two reactive groups link via a medium-length (40 atoms, 46.1 Å), flexible, single molecular weight, discrete polyethylene glycol (dPEG®) linker. The bromoacetyl group reacts chemoselectively with thiols at pH ≥ 8.0. This differs from the maleimide group, which reacts chemoselectively with sulfhydryls within the range of pH 6.5 – 7.5. The DBCO moiety is a strained cyclooctyne ring flanked by two benzyl rings on either side. DBCO was designed for strain promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry.

Specifications

Unit Size 25 mg, 100 mg, 500 mg
Molecular Weight 996.974; single compound
Chemical formula C₄₇H₇₀BrN₃O₁₅
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 40 atoms and 46.1 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, Acetonitrile, DMF, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bromoacetamido-dPEG®₁₂-amido-DBCO appeared first on VectorLabs.

]]>

Description

Bromoacetamido-dPEG®12-amido-DBCO, product number QBD-11223, combines a thiol-reactive bromoacetyl group with dibenzylcyclooctyne (DBCO, also known as DIBAC), a copper-free click chemistry reactive group. The two reactive groups link via a medium-length (40 atoms, 46.1 Å), flexible, single molecular weight, discrete polyethylene glycol (dPEG®) linker. The bromoacetyl group reacts chemoselectively with thiols at pH ≥ 8.0. This differs from the maleimide group, which reacts chemoselectively with sulfhydryls within the range of pH 6.5 – 7.5. The DBCO moiety is a strained cyclooctyne ring flanked by two benzyl rings on either side. DBCO was designed for strain promoted azide-alkyne cycloaddition (SPAAC), also known as copper-free click chemistry.

Specifications

Unit Size25 mg, 100 mg, 500 mg
Molecular Weight996.974; single compound
Chemical formulaC₄₇H₇₀BrN₃O₁₅
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 40 atoms and 46.1 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, Acetonitrile, DMF, DMAC, or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post Bromoacetamido-dPEG®₁₂-amido-DBCO appeared first on VectorLabs.

]]>