Click-&-Go™ DDe Protein Enrichment Kit is an efficient, biotin/streptavidin-free tool for capturing alkyne-modified biomolecules on a cleavable agarose resin via click reaction and its subsequent release under mild conditions. Alkyne-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the cleavable azide agarose resin supplied. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with high stringency virtually eliminating any non-specifically bound proteins. Treatment with 2% hydrazine yields a highly enriched population of nascent proteins. Captured proteins can be protease digested to yield a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis.

The biotin-free enrichment improves signal to noise by eliminating nonspecific binding and increasing selectivity, thus improving detection of low abundant proteins. This approach is fully compatible with widely used MS techniques including iTRAC and ICAT.

The biotin-free, covalent capture approach is superior to biotin or lectin based enrichment approaches, and ideal for proteomics, biomarker discovery, posttranslational modification (PTM) analysis, and more.

Schematic Workflow

Specifications

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Click-&-Go^® Plus Protein Enrichment Kit is an efficient tool for capture of alkyne-modified proteins on picolyl azide-agarose resin. The alkyne-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the picolyl azide-agarose resin supplied. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon protease digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis.

Schematic Workflow

Specifications

The post Click-&-Go® Plus Protein Enrichment Kit appeared first on VectorLabs.

Click-&-Go™ Protein Enrichment Kit is an efficient tool for capture of alkyne-modified proteins on an azide-agarose resin. The alkyne modification can occur via metabolic feeding, enzymatic addition, or chemical modification. The alkyne-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the azide-agarose resin supplied. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon protease digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis.

The biotin-free enrichment improves signal to noise by eliminating nonspecific binding and increasing selectivity, thus improving detection of low abundant proteins. This approach is fully compatible with widely used MS techniques including iTRAC and ICAT.

The biotin-free, covalent capture approach is superior to biotin or lectin based enrichment approaches, and ideal for proteomics, biomarker discovery, posttranslational modification (PTM) analysis, and more.

The Click-&-Go™ Protein Enrichment Kit can be used as direct replacement of Click-iT® Protein Enrichment Kit

Schematic Workflow

Specifications

The post Click-&-Go® Protein Enrichment Kit appeared first on VectorLabs.