Agarose-GLAGLLAR-Alkyne is an agarose resin that is pre-activated with terminal alkyne groups linked to the agarose resin through a short synthetic peptide containing a tryptic cleavage site. The azide-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the Agarose-GLAGLLAR-Alkyne resin. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon tryptic digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis. Importantly, azide-modified targeted peptides can be easily identified by MS analysis, providing a confirmation by a mass shift of 113.05 Da

Specifications

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Agarose-GLAGLLAR-Azide is an agarose resin that is pre-activated with azide groups linked to the agarose resin through a short synthetic peptide containing a tryptic cleavage site. The alkyne-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the Agarose-GLAGLLAR-Azide resin. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon tryptic digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis. Importantly, alkyne-modified targeted peptides can be easily identified by MS analysis, providing a confirmation by a mass shift of 172.10 Da

Specifications

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Picolyl Azide Agarose is a 6% crosslinked agarose resin that is activated with picolyl azide functional groups for covalent capturing alkyne-tagged biomolecules. Picolyl Azide incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boosted the efficiency of the CuAAC reaction resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides was reported (see Selected References).This product is adequate to work in batch or column purification (low pressure).

Specifications

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PC Azide Agarose is a 6% crosslinked agarose resin that is activated with azide groups through a photocleavable linker. Once biomolecules are covalently captures to the resin, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins. Irradiation with a hand-held UV-Vis lamp allows for reagent-less release of captured molecules. This product is adequate to work in batch or column purification (low pressure).

Specifications

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PC Alkyne Agarose is a 6% crosslinked agarose resin that is activated with alkyne groups through a photocleavable linker. Once biomolecules are covalently captures to the resin, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins. Irradiation with a hand-held UV-Vis lamp allows for reagent-less release of captured molecules. This product is adequate to work in batch or column purification (low pressure).

Specifications

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Alkyne agarose is a 6% crosslinked agarose resin that is activated with terminal alkyne functional groups for covalent capturing azide-tagged biomolecules. This product is adequate to work in batch or column purification (low pressure).

Specifications

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DBCO agarose is a 6% crosslinked agarose resin that is activated with DBCO functional groups for covalent capturing azide-tagged biomolecules utilizing Cu-free click reaction. This product is adequate to work in batch or column purification (low pressure).

Specifications

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Methyltetrazine agarose is a 6% crosslinked agarose resin that is activated with methyltetrazine functional groups for covalent immobilization of TCO-modified biomolecules utilizing extremely fast Inverse electron demand Diels–Alder (IEDDA) reaction. This product is ideally suited for preparation of high capacity protein agarose media with almost quantitative capture of proteins.

Specifications

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TCO agarose is a 6% crosslinked agarose resin that is activated with TCO functional groups for covalent immobilization of tetrazine-modified biomolecules utilizing extremely fast Inverse electron demand Diels–Alder (IEDDA) reaction. This product is ideally suited for preparation of high capacity protein agarose media with almost quantitative capture of proteins.

Specifications

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