Cleavable Azide Labeled – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:09:44 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Cleavable Azide Labeled – VectorLabs https://staging.vectorlabs.com 32 32 Agarose-GLAGLLAR-Azide https://staging.vectorlabs.com/products/agarose-glagllar-azide/ Tue, 19 Sep 2023 20:22:58 +0000 https://staging.vectorlabs.com/?post_type=product&p=22685 Description

Agarose-GLAGLLAR-Azide is an agarose resin that is pre-activated with azide groups linked to the agarose resin through a short synthetic peptide containing a tryptic cleavage site. The alkyne-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the Agarose-GLAGLLAR-Azide resin. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon tryptic digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis. Importantly, alkyne-modified targeted peptides can be easily identified by MS analysis, providing a confirmation by a mass shift of 172.10 Da

Specifications

Unit Size 2 ml
Enrichment target
Alkyne-modified proteins
Format
Batch or column purifications
Activation level
5-12 µmol azide groups per mL resin
Bead size Spherical, 50-150 µm
Support 6% Cross-linked agarose
Formulation 50% slurry
Preservative 20% ethanol
Appearance Off-white slurry
Storage 2-8C
Shipping Conditions Ambient temperature

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Description

Agarose-GLAGLLAR-Azide is an agarose resin that is pre-activated with azide groups linked to the agarose resin through a short synthetic peptide containing a tryptic cleavage site. The alkyne-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the Agarose-GLAGLLAR-Azide resin. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon tryptic digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis. Importantly, alkyne-modified targeted peptides can be easily identified by MS analysis, providing a confirmation by a mass shift of 172.10 Da

Specifications

Unit Size2 ml
Enrichment target
Alkyne-modified proteins
Format
Batch or column purifications
Activation level
5-12 µmol azide groups per mL resin
Bead sizeSpherical, 50-150 µm
Support6% Cross-linked agarose
Formulation50% slurry
Preservative20% ethanol
AppearanceOff-white slurry
Storage2-8C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Shao, Y., et al. (2021). Enhancing Comprehensive Analysis of Newly Synthesized Proteins Based on Cleavable Bioorthogonal Tagging. Anal Chem., 93 (27), 9408-9417. [PubMed]

The post Agarose-GLAGLLAR-Azide appeared first on VectorLabs.

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