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Neuronal Tracers

Elucidate anatomical connections of the nervous system

Neuronal tracers provide an opportunity to visualize neural architecture and identify gap junction coupling in vivo or in whole mounts, slice preparations, or cultured cells. Neuronal Tracers can also be used to detect biotin labels using avidin or streptavidin systems (in conjunction with either chromogenic or fluorescence visualization methods).

Dendritic spines, 4h post-labeling with NEUROBIOTIN Plus. Image kindly provided by Dr Judith Schweimer, Dept of Pharmacology, University of Oxford, U.K.
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Fast-spiking interneurons of mouse brain striatum filled with NEUROBIOTIN 488. From Russo G, Nieus TR, Maggi S, and Taverna S. 2013. Dynamics of action potential firing in electrically connected striatal fast-spiking interneurons. Front. Cell. Neurosci. 7:209. doi: 10.3389/fncel.2013.00209


Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents. This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving

For those wanting additional details on the simple protocol and mode of action of the TrueVIEW Autofluorescence Quenching Kit, this brochure contains clear, concise scematics, comparision data with other types of quenchers, and customer testimonials.