These affinity-purified, highly specific antibodies are directed against the most commonly used primary antibody target species and are available with a choice of fluorophores.

Original Fluorophore-Conjugated Secondary Antibodies

All of Vector Laboratories’ fluorophore-conjugated secondary antibodies are affinity-purified and highly cross-adsorbed and are available in a concentrated format. Many are also offered in a convenient ready-to-use format (See VectaFluor™ reagents below):

• Traditional dyes – Fluorescein, Rhodamine, Texas Red^®, AMCA, Phycoerythrin
• Cy^®3 and Cy^®5 dyes – for bright and stable fluorescence and use in a variety of applications
  • Cy^®3 is bright orange with an excitation/emission of 550 nm/570 nm
  • Cy^®5 is a far-red dye with an excitation/emission of 649 nm/670 nm; use it in multiplexing protocols or in super-resolution imaging for its photoswitchable properties
• DyLight^® dyes* – for cell- and tissue-based immunofluorescence and a variety of other applications; offer greater photostability, pH independence, and brighter fluorescence than conventional fluorophores

VectaFluor™ Ready-to-use Antibody Reagents

VectaFluor™ Antibody Reagents include DyLight^® dye-conjugated secondary antibodies as pre-diluted, ready-to-use solutions. Suitable for use with rabbit, mouse, goat, sheep, and bovine IgG primary antibodies, these photostable fluorescence detection reagents allow you to:

• Reduce optimization requirements
• Save time
• Minimize potential dilution errors
• Obtain greater reproducibility in collaborative efforts across lab environments 
• Include ready-to-use 2.5% normal horse serum (15 ml) for blocking.

VectaFluor™ Duet IF Double Labeling Kits

VectaFluor™ Duet IF Double Labeling Kits allow you to apply two colors in one step. These kits save time and effort in double-labeling immunofluorescence (IF) protocols, which can be long and tedious. The kits are formulated to detect a mouse and a rabbit primary antibody — with green and red fluorescence — in a single step.