Product Categories
Enzyme Substrates
Proprietary, easy-to-use enzyme substrates and kits
Vector’s unique Horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme substrates offer:
- Novel color choices — stain antigens in pigmented tissues and melanomas, or localize multiple antigens in a single tissue section
- Visibility under brightfield, darkfield, or electron microscopy
- Unique spectral profiles — useful for antigen colocalization using spectral imaging systems
Our enzyme substrate kits supply the reagents in convenient dropper bottles. This allows you to handle chromogens safely and easily, and eliminates wait times for mixing and dissolving powders or tablets.
Enzyme Substrates / Microscopy Table
This table is designed as a reference to help you determine the optimal mounting medium for your application.
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Type of Microscopy |
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| Substrate | Catalog Number | Brightfield | Darkfield | Electron | Fluorescence | Spectral Imaging |
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Horseradish Peroxidase (HRP) |
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|
DAB (brown) |
SK-4100 |
• |
• |
• |
|
• |
||||
|
DAB-Ni (gray-black) |
SK-4100 |
• |
• |
• |
|
• |
||||
|
ImmPACT DAB (brown) |
SK-4105 |
• |
• |
• |
|
• |
||||
|
ImmPACT DAB EqV (brown) |
SK-4103 |
• |
• |
• |
|
• |
||||
|
Vector VIP (purple) |
SK-4600 |
• |
• |
• |
|
• |
||||
|
ImmPACT VIP (purple) |
SK-4605 |
• |
• |
• |
|
• |
||||
|
SG (blue-gray) |
SK-4700 |
• |
• |
• |
|
• |
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ImmPACT SG (blue-gray) |
SK-4705 |
• |
• |
• |
|
• |
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|
Vector NovaRED (red) |
SK-4800 |
• |
• |
• |
|
• |
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|
ImmPACT NovaRED (red) |
SK-4805 |
• |
• |
• |
|
• |
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|
AEC (red) |
SK-4200 |
• |
|
|
|
• |
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ImmPACT AEC (red) |
SK-4205 |
• |
|
|
|
• |
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ImmPACT AMEC Red (red) |
SK-4285 |
• |
|
|
|
• |
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TMB (blue) |
SK-4400 |
• |
|
|
|
• |
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Alkaline Phosphatase (AP) |
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|
Vector Red (magenta) |
SK-5100 |
• |
|
|
• |
• |
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ImmPACT Vector Red (magenta) |
SK-5105 |
• |
|
|
• |
• |
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|
Vector Black (black) |
SK-5200 |
• |
|
|
|
|
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Vector Blue (blue) |
SK-5300 |
• |
|
|
• |
• |
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|
BCIP / NBT (indigo) |
SK-5400 |
• |
|
|
• |
• |
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Additional Enzyme Substrate Characteristics
This table is designed as a reference to help you determine the optimal mounting medium for your application.
| Substrate | Catalog Number | Mounting Medium |
Contrast in Pigmented Tissue |
Multiple Labelling |
Heat Resistant |
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|
Horseradish Peroxidase (HRP) |
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|
DAB (brown) |
SK-4100 |
Non-aqueous |
|
• |
• |
|||||
|
DAB-Ni (gray-black) |
SK-4100 |
Non-aqueous |
|
• |
|
|||||
|
ImmPACT DAB (brown) |
SK-4105 |
Non-aqueous |
|
• |
• |
|||||
|
ImmPACT DAB EqV (brown) |
SK-4103 |
Non-aqueous |
|
• |
• |
|||||
|
Vector VIP (purple) |
SK-4600 |
Non-aqueous |
• |
• |
|
|||||
|
ImmPACT VIP (purple) |
SK-4605 |
Non-aqueous |
• |
• |
|
|||||
|
SG (blue-gray) |
SK-4700 |
Non-aqueous |
• |
• |
|
|||||
|
ImmPACT SG (blue-gray) |
SK-4705 |
Non-aqueous |
• |
• |
|
|||||
|
Vector NovaRED (red) |
SK-4800 |
Non-aqueous |
• |
• |
|
|||||
|
ImmPACT NovaRED (red) |
SK-4805 |
Non-aqueous |
• |
• |
|
|||||
|
AEC (red) |
SK-4200 |
Aqueous |
• |
• |
|
|||||
|
ImmPACT AEC (red) |
SK-4205 |
Aqueous |
• |
• |
|
|||||
|
ImmPACT AMEC Red (red) |
SK-4285 |
Aqueous |
• |
• |
|
|||||
|
TMB (blue) |
SK-4400 |
Non-Aqueous |
• |
• |
|
|||||
|
Alkaline Phosphatase (AP) |
||||||||||
|
Vector Red (magenta) |
SK-5100 |
Non-Aqueous |
• |
• |
• |
|||||
|
ImmPACT Vector Red (magenta) |
SK-5105 |
Non-Aqueous |
• |
• |
• |
|||||
|
Vector Black (black) |
SK-5200 |
Non-Aqueous |
|
|
|
|||||
|
Vector Blue (blue) |
SK-5300 |
Non-Aqueous |
• |
• |
• |
|||||
|
BCIP / NBT (indigo) |
SK-5400 |
Non-Aqueous |
|
• |
• |
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Explore Enzyme Substrates (Chromogens)
Vector Labs offers alkaline phosphatase (AP) substrates suitable for several applications including cell and tissue based staining (IHC, IF, ISH), ELISA’s and blotting.
Vector Labs offers peroxidase (HRP) substrates suitable for several applications including cell and tissue based staining (IHC, ISH) and blotting.
Resources
A valuable resource for those considering localizing two or more antigens in the same tissue section. Protocols for chromogenic detection are accompanied by schematic illustrations of the procedures.
A chart of all pair-wise combinations of substrates is included along with photographic examples. Practical tips regarding order of substrates, blocking, and proper controls are also provided.
- In addition to photomicrographs that help the user visualize the color that is generally produced
by each substrate, this brochure contains information on substrate properties that are especially useful for those who wish to multiplex (or introduce multiple labels in the same tissue section): A table of substrate properties, a schematic of relative senstitivities of substrate formulations, and a table of pairwise substrate compatibility are also included.hiving
