Antigen Unmasking for Immunofluorescence and Immunohistochemistry workflows Unlock Epitope Accessibility for Optimal Immunostaining Formalin fixation is a standard method in immunohistochemistry (IHC) and immunofluorescence (IF) workflows to preserve tissue morphology. However, formalin induces cross-linking of proteins which can mask antigenic sites and hinder antibody binding.   Antigen unmasking techniques restore epitope accessibility. The most common approach … Continue reading Antigen Unmasking